Characterization of Causative Allergens for Wheat-Dependent Exercise-Induced Anaphylaxis Sensitized with Hydrolyzed Wheat Proteins in Facial Soap
-
- Yokooji Tomoharu
- Department of Pathophysiology and Therapeutics, Graduate School of Biomedical and Health Sciences, Hiroshima University Faculty of Pharmaceutical Sciences, Hiroshima University
-
- Kurihara Saki
- Faculty of Pharmaceutical Sciences, Hiroshima University
-
- Murakami Tomoko
- Faculty of Pharmaceutical Sciences, Hiroshima University
-
- Chinuki Yuko
- Department of Dermatology, Faculty of Medicine, Shimane University
-
- Takahashi Hitoshi
- Department of Dermatology, Faculty of Medicine, Shimane University
-
- Morita Eishin
- Department of Dermatology, Faculty of Medicine, Shimane University
-
- Harada Susumu
- Harada Skin Clinic
-
- Ishii Kaori
- Department of Dermatology, Graduate School of Biomedical and Health Sciences, Hiroshima University
-
- Hiragun Makiko
- Department of Dermatology, Graduate School of Biomedical and Health Sciences, Hiroshima University
-
- Hide Michihiro
- Department of Dermatology, Graduate School of Biomedical and Health Sciences, Hiroshima University
-
- Matsuo Hiroaki
- Department of Pathophysiology and Therapeutics, Graduate School of Biomedical and Health Sciences, Hiroshima University Faculty of Pharmaceutical Sciences, Hiroshima University
Search this article
Abstract
Background: In Japan, hydrolyzed wheat proteins (HWP) have been reported to cause wheat-dependent exercise-induced anaphylaxis (WDEIA) by transcutaneous sensitization using HWP-containing soap. Patients develop allergic reactions not only with soap use, but also with exercise after the intake of wheat protein (WP). ω5-Gliadin and HMW-glutenin were identified as major allergens in conventional WP-WDEIA patients. However, the allergens in HWP-WDEIA have yet to be elucidated.<br> Methods: Sera were obtained from 22 patients with HWP-sensitized WDEIA. The allergenic activities of HWP and six recombinant wheat gluten proteins, including α/β-, γ-, ω1,2- and ω5-gliadin and low- and high molecular weight (HMW)-glutenins, were characterized by immunoblot analysis and histamine releasing test. IgE-binding epitopes were identified using arrays of overlapping peptides synthesized on SPOTs membrane.<br> Results: Immunoblot analysis showed that IgE antibodies (Abs) from HWP-WDEIA bound to α/β-, γ- and ω1,2-gliadin. Recombinant γ-gliadin induced significant histamine release from basophils in eight of 11 patients with HWP-WDEIA. An IgE-binding epitope "QPQQPFPQ" was identified within the primary sequence of γ-gliadin, and the deamidated peptide containing the "PEEPFP" sequence bound with IgE Abs more strongly compared to the native epitope-peptide. The epitope-peptide inhibited IgE-binding to HWP, indicating that the specific IgE to HWP cross-reacts with γ-gliadin.<br> Conclusions: HWP-WDEIA patients could be sensitized to HWP containing a PEEPFP sequence, and WDEIA symptoms after WP ingestion could partly be induced by γ-gliadin. These findings could be useful to help develop tools for diagnosis and desensitization therapy for HWP-WDEIA.<br>
Journal
-
- Allergology International
-
Allergology International 62 (4), 435-445, 2013
Japanese Society of Allergology