Enhanced Glucose Requirement in Human Hepatoma-derived HuH-7 Cells by Forced Expression of the bcl-2 Gene

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Author(s)

    • Okamoto Kyoko
    • Molecular & Cellular Biology, Graduate School of Human Health Sciences, Siebold University of Nagasaki
    • Muraguchi Takashi
    • Molecular & Cellular Biology, Graduate School of Human Health Sciences, Siebold University of Nagasaki
    • Shidoji Yoshihiro
    • Molecular & Cellular Biology, Graduate School of Human Health Sciences, Siebold University of Nagasaki

Abstract

To explore the metabolic effects of Bcl-2 in tumor cells, a stable clone of HuH-7/bcl-2 and its control HuH-7/neo were established. Mitochondrial localization of ectopic Bcl-2 was demonstrated both by western blotting and immunofluorescence. HuH-7/bcl-2 cells consumed glucose at a higher rate, exhausted the available cellular ATP and died on day 9, while HuH-7/neo cells were still alive for 10 days under the same condition where cells were cultured without replenishment of the medium. The expression of the <i>hexokinase II</i> gene was up-regulated in HuH-7/bcl-2 at its protein level. Taken together, we suggest that the forced expression of Bcl-2 in human hepatoma may cause the cells to become more glucose-dependent for survival.<br>

Journal

  • Journal of Clinical Biochemistry and Nutrition

    Journal of Clinical Biochemistry and Nutrition 43(2), 101-108, 2008

    SOCIETY FOR FREE RADICAL RESEARCH JAPAN

Codes

  • NII Article ID (NAID)
    130004879136
  • Text Lang
    ENG
  • ISSN
    0912-0009
  • Data Source
    J-STAGE 
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