Lansoprazole inhibits mitochondrial superoxide production and cellular lipid peroxidation induced by indomethacin in RGM1 cells
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- Rai Kanho
- Graduate School of Comprehensive Human Sciences, University of Tsukuba
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- Matsui Hirofumi
- Graduate School of Comprehensive Human Sciences, University of Tsukuba
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- Kaneko Tsuyoshi
- Graduate School of Comprehensive Human Sciences, University of Tsukuba
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- Nagano Yumiko
- Graduate School of Comprehensive Human Sciences, University of Tsukuba
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- Shimokawa Osamu
- Graduate School of Comprehensive Human Sciences, University of Tsukuba
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- Udo Jumpei
- Graduate School of Comprehensive Human Sciences, University of Tsukuba
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- Hirayama Aki
- The Center of Integrative Medicine, Tsukuba University of Technology
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- Hyodo Ichinosuke
- Graduate School of Comprehensive Human Sciences, University of Tsukuba
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- Indo Hiroko P.
- The Department of Oncology and Department of Space Environmental Medicine, Kagoshima University Graduate School of Medical and Dental Sciences
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- Majima Hideyuki J.
- The Department of Oncology and Department of Space Environmental Medicine, Kagoshima University Graduate School of Medical and Dental Sciences
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Abstract
Lansoprazole is effective in healing non-steroidal anti-inflammatory drugs induced ulcers, and antioxidant properties have been thought to play a key role in healing ulcers. We hypothesize that lansoprazole exerts a cytoprotective effect by inhibiting reactive oxygen species leakage from mitochondria and lipid peroxidation. We pretreated gastric epithelial RGM1 cells with lansoprazole and then treated them with indomethacin in vitro. We found that the lansoprazole pretreatment significantly reduced cellular injury, maintained mitochondrial transmembrane potential, and decreased lipid peroxidation. Furthermore, the signal intensity of the electron spin resonance spectrum of the indomethacin-treated mitochondria which were pretreated with lansoprazole showed considerable reduction compared to those without the lansoprazole pretreatment. These results suggest that lansoprazole reduced superoxide production in the mitochondria of indomethacin treated cells, and subsequently inhibited lipid peroxide and cellular injury in gastric epithelial cells.<br>
Journal
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- Journal of Clinical Biochemistry and Nutrition
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Journal of Clinical Biochemistry and Nutrition 49 (1), 25-30, 2011
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