Protective effects of fermented rice vinegar sediment (Kurozu moromimatsu) in a diethylnitrosamine-induced hepatocellular carcinoma animal model

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Abstract

<i>Kurozu moromimatsu</i> is the sediment of <i>Kurozu</i>, a jar-fermented Japanese black vinegar produced from unpolished rice. Here, we examined the protective effects of <i>Kurozu moromimatsu</i> in a diethylnitrosamine-induced model of hepatocellular carcinoma. Thirty-two F344 rats were divided into two groups; the control group received basal CE-2 diet, and the <i>Kurozu moromimatsu</i> group received CE-2 diet containing <i>Kurozu moromimatsu</i>. At 16 weeks after initial intraperitoneal administration of diethylnitrosamine (150 mg/kg/week), serum was collected from half the rats. These rats were sacrificed and the liver was resected for histological examination of hematoxylin-eosin-stained sections and assay of matrix metalloproteinase-2 and matrix metalloproteinase-9 levels in tumor tissues. Glutathione S-transferase placental form-positive foci were evaluated by immunostaining for glutathione S-transferase placental form. The remaining rats were maintained for evaluation of survival. There were no significant differences of serum transaminases, tumor necrosis factor-alpha, and also no marked hepatic histological differences, between the two groups. However, the size of hepatocellular carcinomas was greatly decreased and the levels of activated matrix metalloproteinase-2 and -9 were significantly reduced in the <i>Kurozu moromimatsu</i> group. Further, survival was significantly prolonged in the <i>Kurozu moromimatsu</i> group compared with the control. These results indicate that <i>Kurozu moromimatsu</i> inhibited the growth of hepatocellular carcinoma.<br>

Journal

  • Journal of Clinical Biochemistry and Nutrition

    Journal of Clinical Biochemistry and Nutrition 49(1), 31-35, 2011

    SOCIETY FOR FREE RADICAL RESEARCH JAPAN

Codes

  • NII Article ID (NAID)
    130004879259
  • Text Lang
    ENG
  • ISSN
    0912-0009
  • Data Source
    J-STAGE 
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