Up-regulation of stanniocalcin 1 expression by 1,25-dihydroxy vitamin D<sub>3</sub> and parathyroid hormone in renal proximal tubular cells
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- Hung Nguyen Trong
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
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- Yamamoto Hironori
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
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- Takei Yuichiro
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
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- Masuda Masashi
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
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- Otani Ayako
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
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- Kozai Mina
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
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- Ikeda Shoko
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
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- Nakahashi Otoki
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
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- Tanaka Sarasa
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
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- Taketani Yutaka
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
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- Takeda Eiji
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
Bibliographic Information
- Other Title
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- Up-regulation of stanniocalcin 1 expression by 1,25-dihydroxy vitamin D3 and parathyroid hormone in renal proximal tubular cells
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Abstract
Stanniocalcin 1 and stanniocalcin 2 are two glycoprotein hormones, which act as calcium phosphate-regulating factor on intestine and kidney. We have previously reported that stanniocalcin 2 expression is positively and negatively controlled by 1,25(OH)2D3 and parathyroid hormone in renal proximal tubular cells. However, it has been unclear whether they regulate the stanniocalcin 1 gene expression. In this study, we identified the opossum stanniocalcin 1 cDNA sequence. The opossum stanniocalcin 1 amino acid sequence had 83% homology with human stanniocalcin 1, and has a conserved putative N-linked glycosylation site. Real-time PCR analysis using opossum kidney proximal tubular (OK-P) cells revealed that the mRNA levels of stanniocalcin 1 gene is up-regulated by both 1,25(OH)2D3 and parathyroid hormone in dose-dependent and time-dependent manners. We also demonstrated that the stanniocalcin 1 expression was increased in parathyroid hormone injected rat kidney. Furthermore, the mRNA expression of stanniocalcin 1 and stanniocalcin 2 were oppositely regulated by phorbol 12,13-myristic acetate, a specific PKC activator. Interestingly, the up-regulation of stanniocalcin 1 gene by 1,25(OH)2D3 and phorbol 12,13-myristic acetate were not prevented in the presence of actinomycin D, an RNA synthesis inhibitor. These results suggest that the stanniocalcin 1 gene expression is up-regulated by 1,25(OH)2D3 and parathyroid hormone through mRNA stabilization in renal proximal tubular cells.<br>
Journal
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- Journal of Clinical Biochemistry and Nutrition
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Journal of Clinical Biochemistry and Nutrition 50 (3), 227-233, 2012
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