Dietary phosphate restriction ameliorates endothelial dysfunction in adenine-induced kidney disease rats
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- Van Tan Vu
- Department of Clinical Nutrition, University of Tokushima Graduate School
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- Watari Eriko
- Department of Clinical Nutrition, University of Tokushima Graduate School
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- Taketani Yutaka
- Department of Clinical Nutrition, University of Tokushima Graduate School
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- Kitamura Tomoyo
- Department of Clinical Nutrition, University of Tokushima Graduate School
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- Shiota Asuka
- Department of Clinical Nutrition, University of Tokushima Graduate School
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- Tanaka Terumi
- Department of Clinical Nutrition, University of Tokushima Graduate School
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- Tanimura Ayako
- Department of Clinical Nutrition, University of Tokushima Graduate School
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- Harada Nagakatsu
- Department of Nutrition and Metaboism, University of Tokushima Graduate School
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- Nakaya Yutaka
- Department of Nutrition and Metaboism, University of Tokushima Graduate School
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- Yamamoto Hironori
- Department of Clinical Nutrition, University of Tokushima Graduate School
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- Miyamoto Ken-ichi
- Department of Molecular Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
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- Takeda Eiji
- Department of Clinical Nutrition, University of Tokushima Graduate School
Abstract
Hyperphosphatemia causes endothelial dysfunction as well as vascular calcification. Management of serum phosphate level by dietary phosphate restriction or phosphate binders is considered to be beneficial to prevent chronic kidney disease patients from cardiovascular disease, but it has been unclear whether keeping lower serum phosphate level can ameliorate endothelial dysfunction. In this study we investigated whether low-phosphate diet can ameliorate endothelial dysfunction in adenine-induced kidney disease rats, one of useful animal model of chronic kidney disease. Administration of 0.75% adenine-containing diet for 21 days induced renal failure with hyperphosphatemia, and impaired acetylcholine-dependent vasodilation of thoracic aortic ring in rats. Then adenine-induced kidney disease rats were treated with either control diet (1% phosphate) or low-phosphate diet (0.2% phosphate) for 16 days. Low-phosphate diet ameliorated not only hyperphosphatemia but also the impaired vasodilation of aorta. In addition, the activatory phosphorylation of endothelial nitric oxide synthase at serine 1177 and Akt at serine 473 in the aorta were inhibited by in adenine-induced kidney disease rats. The inhibited phosphorylations were improved by the low-phosphate diet treatment. Thus, dietary phosphate restriction can improve aortic endothelial dysfunction in chronic kidney disease with hyperphosphatemia by increase in the activatory phosphorylations of endothelial nitric oxide synthase and Akt.<br>
Journal
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- Journal of Clinical Biochemistry and Nutrition
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Journal of Clinical Biochemistry and Nutrition 51 (1), 27-32, 2012
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