Short-term dietary phosphate restriction up-regulates ileal fibroblast growth factor 15 gene expression in mice
-
- Nakahashi Otoki
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
-
- Yamamoto Hironori
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School Department of Health and Nutrition, Faculty of Human Life, Jin-ai University Division of Nephrology, Department of General Medicine, Faculty of Medical Sciences, University of Fukui
-
- Tanaka Sarasa
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
-
- Kozai Mina
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
-
- Takei Yuichiro
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
-
- Masuda Masashi
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
-
- Kaneko Ichiro
- Department of Molecular Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
-
- Taketani Yutaka
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
-
- Iwano Masayuki
- Division of Nephrology, Department of General Medicine, Faculty of Medical Sciences, University of Fukui
-
- Miyamoto Ken-ichi
- Department of Molecular Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
-
- Takeda Eiji
- Department of Clinical Nutrition, Institute of Health Biosciences, University of Tokushima Graduate School
Search this article
Abstract
Members of the fibroblast growth factor (FGF) 19 subfamily, including FGF23, FGF15/19, and FGF21, have a role as endocrine factors which influence the metabolism of inorganic phosphate (Pi) and vitamin D, bile acid, and energy. It has been reported that dietary Pi regulates circulating FGF23. In this study, the short-term effects of dietary Pi restriction on the expression of FGF19 subfamily members in mice were analyzed. An initial analysis confirmed plasma FGF23 levels positively correlated with the amount of dietary Pi. On the other hand, ileal Fgf15 gene expression, but not hepatic Fgf21 gene expression, was up-regulated by dietary Pi restriction. In addition, we observed the increase of plasma 1,25-dihydroxyvitamin D [1,25(OH)2D] levels by dietary Pi restriction, and the up-regulation of ileal Fgf15 mRNA expression by 1,25(OH)2D3 and vitamin D receptor (VDR). Importantly, dietary Pi restriction-induced Fgf15 gene expression was prevented in VDR-knockout mice. Furthermore, diurnal variations of plasma triglyceride concentrations and hepatic mRNA expression of the bile acid synthesis enzyme Cyp7a1 as one of Fgf15 negative target genes was influenced by dietary Pi restriction. These results suggest that dietary Pi restriction up-regulates ileal Fgf15 gene expression through 1,25(OH)2D3 and VDR, and may affect hepatic bile acid homeostasis.
Journal
-
- Journal of Clinical Biochemistry and Nutrition
-
Journal of Clinical Biochemistry and Nutrition 54 (2), 102-108, 2014
SOCIETY FOR FREE RADICAL RESEARCH JAPAN