Sesamol suppresses cyclooxygenase-2 transcriptional activity in colon cancer cells and modifies intestinal polyp development in <i>Apc</i><sup>Min/+</sup> mice
-
- Shimizu Satomi
- Division of Cancer Prevention Research, National Cancer Center Research Institute Faculty of Life Sciences, Toyo University
-
- Fujii Gen
- Division of Cancer Prevention Research, National Cancer Center Research Institute
-
- Takahashi Mami
- Central Animal Division, National Cancer Center Research Institute
-
- Nakanishi Ruri
- Division of Cancer Prevention Research, National Cancer Center Research Institute
-
- Komiya Masami
- Division of Cancer Prevention Research, National Cancer Center Research Institute
-
- Shimura Misato
- Division of Cancer Prevention Research, National Cancer Center Research Institute School of Pharmaceutical Sciences, Tokyo University of Science
-
- Noma Nobuharu
- Division of Cancer Prevention Research, National Cancer Center Research Institute School of Pharmaceutical Sciences, Tokyo University of Science
-
- Onuma Wakana
- Division of Cancer Prevention Research, National Cancer Center Research Institute School of Pharmaceutical Sciences, Tokyo University of Science
-
- Terasaki Masaru
- Faculty of Pharmaceutical Sciences, Health Science University of Hokkaido
-
- Yano Tomohiro
- Faculty of Life Sciences, Toyo University
-
- Mutoh Michihiro
- Division of Cancer Prevention Research, National Cancer Center Research Institute
Bibliographic Information
- Other Title
-
- Sesamol suppresses cyclooxygenase-2 transcriptional activity in colon cancer cells and modifies intestinal polyp development in ApcMin/+ mice
Search this article
Abstract
Excessive prostaglandin production by cyclooxygenase-2 in stromal and epithelial cells is a causative factor of colorectal carcinogenesis. Thus, compounds which inhibit cyclooxygenase-2 transcriptional activity in colon epithelial cells could be candidates for anti-carcinogenic agents. A cyclooxygenase-2 transcriptional activity in the human colon cancer cell line DLD-1 has been measured using a β-galactosidase reporter gene system. Using this system, we demonstrated that the decrease in basal cyclooxygenase-2 transcriptional activities at 100 µM sesamol, one of the lignans in sesame seeds, was 50%. Other compounds in sesame seeds such as sesamin, sesamolin, ferulic acid, and syringic acid did not exhibit significant suppression of cyclooxygenase-2 transcriptional activity at up to 100 µM. In a following experiment, 6-week-old male Min mice, Apc-deficient mice, were divided into a non-treated and 500 ppm sesamol groups. At the age of 15 weeks, it was found that treatment with sesamol decreased the number of polyps in the middle part of small intestine to 66.1% of the untreated value. Moreover, sesamol suppressed cyclooxygenase-2 and cytosolic prostaglandin E2 synthase mRNA in the polyp parts. The present findings may demonstrate the novel anti-carcinogenetic property of sesamol, and imply that agents that can suppress cyclooxygenase-2 expression may be useful cancer chemopreventive agents.
Journal
-
- Journal of Clinical Biochemistry and Nutrition
-
Journal of Clinical Biochemistry and Nutrition 54 (2), 95-101, 2014
SOCIETY FOR FREE RADICAL RESEARCH JAPAN