Involvement of NADPH oxidases in suppression of cyclooxygenase-2 promoter-dependent transcriptional activities by sesamol

• Shimizu Satomi

  Division of Cancer Prevention Research, National Cancer Center Research Institute Graduate School of Life Sciences, Toyo University

• Ishigamori Rikako

  Division of Cancer Prevention Research, National Cancer Center Research Institute

• Fujii Gen

  Division of Cancer Prevention Research, National Cancer Center Research Institute

• Takahashi Mami

  Central Animal Division, National Cancer Center Research Institute

• Onuma Wakana

  Division of Cancer Prevention Research, National Cancer Center Research Institute School of Pharmaceutical Sciences, Tokyo University of Science

• Terasaki Masaru

  Department of Health and Environmental Sciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Yano Tomohiro

  Graduate School of Life Sciences, Toyo University

• Mutoh Michihiro

  Division of Cancer Prevention Research, National Cancer Center Research Institute

Abstract

Cyclooxygenase-2 (COX-2) has been shown to play an important role in colon carcinogenesis. Moreover, one of the components of reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, NADPH oxidase 1 (NOX1), dominantly expressed in the colon, is implicated in the pathogenesis of colon cancer. We have reported that sesamol, one of the lignans in sesame seeds, suppressed COX-2 gene transcriptional activity in human colon cancer cells, and also suppressed intestinal polyp formation in Apc-mutant mice. In the present study, we investigated the involvement of NADPH oxidase in the inhibition of COX-2 transcriptional activity by sesamol. We found that several NADPH oxidase inhibitors, such as apocynin, showed suppressive effects on COX-2 transcriptional activity. Moreover, sesamol significantly suppressed NOX1 mRNA levels in a dose-dependent manner. In addition, we demonstrated that knockdown of NOX1 successfully suppressed COX-2 transcriptional activity. These results suggest that inhibition of NADPH oxidase, especially NOX1, may be involved in the mechanism of the suppression of COX-2 transcriptional activity by sesamol.

Journal

• Journal of Clinical Biochemistry and Nutrition

  Journal of Clinical Biochemistry and Nutrition 56 (2), 118-122, 2015

  SOCIETY FOR FREE RADICAL RESEARCH JAPAN

Keywords

• cyclooxygenase-2
• NADPH oxidase
• sesame
• sesamol
• colon cancer

Details

• CRID: 1390001204674009856
• NII Article ID: 130004879380
• DOI: 10.3164/jcbn.14-89
• ISSN: 18805086
• ISSN: 09120009
• Web Site: https://search.jamas.or.jp/link/ui/2016045664
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed