Effect of Probucol on the Measurement of Lipid Peroxide

J-STAGE
  • TANI Takeshi
    Tokushima Research Institute, Otsuka Pharmaceutical Co., Ltd.
  • NAGATA Yasuo
    Saga Research Institute, Otsuka Pharmaceutical Co., Ltd.

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Other Title
  • プロブコールの過酸化脂質測定に与える影響

Abstract

It is well known that probucol has an antioxidant property and that it inhibits LDL oxidation. Nevertheless, it was reported that when measured by the TBA method the administration of probucol increased serum lipid peroxide. In this study, the effect of probucol on the measurement of lipid peroxide, using the TBA method in particular, was examined. Adding probucol to serum increased the TBA reactive substance (TBARS) in a concentration-dependent manner, but did not affect lipid hydroperoxide by the method with methylene-blue derivative; the greater the volume of serum taken, the higher the TBARS concentration that was found in the presence of probucol. The increased TBAR was dependent on the concentration of probucol in the TBA reaction mixture, but independent of the concentration of probucol in the serum. Furthermore, TBARS values were greatly affected by a lower pH in the presence of probucol. These results suggest that the TBA method overstimates lipid peroxide in serum that contains probucol. Therefore, special care is required when TBARS is used as an index of lipid peroxide in biological samples.

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