A novel <i>GmFAD3-2a</i> mutant allele developed through TILLING reduces α-linolenic acid content in soybean seed oil

• Hoshino Tomoki

  Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Yamagata University

• Watanabe Satoshi

  Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University

• Takagi Yutaka

  Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University

• Anai Toyoaki

  Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University

Bibliographic Information

Other Title

• A novel GmFAD3-2a mutant allele developed through TILLING reduces α-linolenic acid content in soybean seed oil
• A novel GmFAD3-2a mutant allele developed through TILLING reduces alpha-linolenic acid content in soybean seed oil

Abstract

Soybean (Glycine max (L.) Merr.) oil typically contains 8% α-linolenic acid that is highly unstable and easily oxidized. This property is undesirable in many food and industrial applications. Genetic strategies for reducing α-linolenic acid content would enhance the commercial value. However, genetic resources for low α-linolenic acid content are limited among natural soybean variations. Microsomal omega-3-fatty acid desaturase (FAD3) is responsible for the synthesis of α-linolenic acid in the polyunsaturated fatty acid pathway. There are four FAD3 homologs (Glyma02g39230, Glyma11g27190, Glyma14g37350 and Glyma18g06950) in the soybean genome. While non-functional alleles have been reported for Glyma02g39230 (GmFAD3-1a) and Glyma14g37350 (GmFAD3-1b), little variation is seen in Glyma18g06950 (GmFAD3-2a). We isolated seven mutant GmFAD3-2a alleles, each containing a single-nucleotide substitution, from 39,100 independent mutant lines by using targeting induced local lesions in genomes (TILLING). Analysis of GmFAD3-2a transcripts and enzyme activities revealed that one missense mutant, ‘P1-A9’, contains a non-functional allele of GmFAD3-2a. By combining three non-functional alleles (GmFAD3-1a, GmFAD3-1b, and GmFAD3-2a), we generated soybean lines containing <2% α-linolenic acid in their seeds. The reverse-genetics-based development of novel mutant alleles in the fatty acid metabolic pathway will allow the improvement of soybean with better oil quality through conventional breeding.

Journal

• Breeding Science

  Breeding Science 64 (4), 371-377, 2014

  Japanese Society of Breeding

Keywords

• α-linolenic acid
• reverse genetics
• soybean oil quality
• TILLING

Details

• CRID: 1390001204723561600
• NII Article ID: 130004888870
• NII Book ID: AA11317194
• DOI: 10.1270/jsbbs.64.371
• ISSN: 13473735
• ISSN: 13447610
• NDL BIB ID: 026057257
• Web Site: http://id.ndl.go.jp/bib/026057257
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026057257
• Web Site: https://www.jstage.jst.go.jp/article/jsbbs/64/4/64_371/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed