A novel <i>GmFAD3-2a</i> mutant allele developed through TILLING reduces α-linolenic acid content in soybean seed oil
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- Hoshino Tomoki
- Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Yamagata University
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- Watanabe Satoshi
- Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University
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- Takagi Yutaka
- Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University
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- Anai Toyoaki
- Laboratory of Plant Genetics and Breeding, Faculty of Agriculture, Saga University
Bibliographic Information
- Other Title
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- A novel GmFAD3-2a mutant allele developed through TILLING reduces α-linolenic acid content in soybean seed oil
- A novel GmFAD3-2a mutant allele developed through TILLING reduces alpha-linolenic acid content in soybean seed oil
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Abstract
Soybean (Glycine max (L.) Merr.) oil typically contains 8% α-linolenic acid that is highly unstable and easily oxidized. This property is undesirable in many food and industrial applications. Genetic strategies for reducing α-linolenic acid content would enhance the commercial value. However, genetic resources for low α-linolenic acid content are limited among natural soybean variations. Microsomal omega-3-fatty acid desaturase (FAD3) is responsible for the synthesis of α-linolenic acid in the polyunsaturated fatty acid pathway. There are four FAD3 homologs (Glyma02g39230, Glyma11g27190, Glyma14g37350 and Glyma18g06950) in the soybean genome. While non-functional alleles have been reported for Glyma02g39230 (GmFAD3-1a) and Glyma14g37350 (GmFAD3-1b), little variation is seen in Glyma18g06950 (GmFAD3-2a). We isolated seven mutant GmFAD3-2a alleles, each containing a single-nucleotide substitution, from 39,100 independent mutant lines by using targeting induced local lesions in genomes (TILLING). Analysis of GmFAD3-2a transcripts and enzyme activities revealed that one missense mutant, ‘P1-A9’, contains a non-functional allele of GmFAD3-2a. By combining three non-functional alleles (GmFAD3-1a, GmFAD3-1b, and GmFAD3-2a), we generated soybean lines containing <2% α-linolenic acid in their seeds. The reverse-genetics-based development of novel mutant alleles in the fatty acid metabolic pathway will allow the improvement of soybean with better oil quality through conventional breeding.
Journal
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- Breeding Science
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Breeding Science 64 (4), 371-377, 2014
Japanese Society of Breeding
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Details 詳細情報について
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- CRID
- 1390001204723561600
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- NII Article ID
- 130004888870
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- NII Book ID
- AA11317194
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- ISSN
- 13473735
- 13447610
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- NDL BIB ID
- 026057257
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed