Cytosolic Ca2+ alteration mediates both ryanodine receptor and IP3 receptor in TE671/RD cells
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- EDAHIRO Shigeki
- Department of Neurology and Neurobiology of Aging, Kanazawa University Graduate School of Medical Science
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- YOSHIKAWA Hiroaki
- Department of Neurology and Neurobiology of Aging, Kanazawa University Graduate School of Medical Science Health Service Center Kanazawa University
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- IWASA Kazuo
- Department of Neurology and Neurobiology of Aging, Kanazawa University Graduate School of Medical Science
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- HASHII Minako
- Department of Biophysical Genetics, Kanazawa University Graduate School of Medical Science
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- YAMADA Masahito
- Department of Neurology and Neurobiology of Aging, Kanazawa University Graduate School of Medical Science
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Abstract
TE671/RD is a cell line obtained from the rhabdomyosarcoma cells. In the present study, we examined [Ca2+]i alteration induced by acetylcholine (ACh) in TE671/RD cells with the special attention to ryanodine receptor (RyR) and inositol 1, 4, 5-trisphosphate receptor (IP3R). The change of [Ca2+]i was mediated by muscarinic type 3 (m3) AChR. Both phosphatidylinositol-specific phospholipase C blocker (U73122) and IP3R blocker (2-APB) inhibited ACh-induced [Ca2+]i elevation, suggesting that IP3 pathway is involved in [Ca2+]i alteration. Ryanodine and FK506 increased ACh-induced [Ca2+]i elevation, which was decreased by RyR blocker (ruthenium red). These results suggest that RyR and FK506 binding protein 12 kDa (FKBP12) complex was involved in [Ca2+]i change, and that TE671/RD cell line has a hybrid characteristic of smooth and skeletal muscles.
Journal
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- Biomedical Research
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Biomedical Research 25 (6), 255-261, 2004
Biomedical Research Press
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Details 詳細情報について
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- CRID
- 1390001204902113152
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- NII Article ID
- 130004903682
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- NII Book ID
- AA00110128
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- COI
- 1:CAS:528:DC%2BD2MXpsFentQ%3D%3D
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- ISSN
- 1880313X
- 03886107
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed