Behavior of osteoblast-like cells on fibronectin or BMP-2 immobilized surface

• MATSUZAKA Kenichi

  Department of Clinical Pathophysiology and Oral Health Science Center

• YOSHINARI Masao

  Department of Dental Materials Science and Oral Health Science Center

• KOKUBU Eitoyo

  Department of Clinical Pathophysiology and Oral Health Science Center

• SHIMONO Masaki

  Department of Pathology and Oral Health Science Center, Tokyo Dental College

• INOUE Takashi

  Department of Clinical Pathophysiology and Oral Health Science Center

Abstract

Property modification of an implant surface is known to influence the behavior of cells surrounding implant material. This study examined the osteogenic cell behavior on protein immobilized surface in vitro. Following plasma surface modification, fibronectin or bone morphogenetic protein-2 (BMP-2) was immobilized. The number of cells adhering to fibronectin-immobilized surface increased after 1 and 2 h of incubation compared with non-immobilized surface. Alkaline phosphatase activity and osteocalcin mRNA expression of the osteogenic cells on the BMP-2 immobilized surface was greater than that on the non-immobilized surface. This study demonstrated that protein can be immobilized to a polystyrene surface after treatment with O₂ plasma and that the osteogenic cells surrounding a biomaterial can be controlled by the immobilization of protein to the biomaterial.

Journal

• Biomedical Research

  Biomedical Research 25 (6), 263-268, 2004

  Biomedical Research Press

Keywords

• General Biochemistry, Genetics and Molecular Biology
• General Medicine

Details

• CRID: 1390001204902116864
• NII Article ID: 130004903683
• NII Article ID: 80017125651
• DOI: 10.2220/biomedres.25.263
• COI: 1:CAS:528:DC%2BD2MXpsFenug%3D%3D
• ISSN: 1880313X
• ISSN: 03886107
• Web Site: https://search.jamas.or.jp/link/ui/2006149887
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed