Relationship between endothelin-1 and interleukin-1.BETA. in inflamed periodontal tissues
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- Rikimaru Tetsuya
- Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College
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- Awano Shuji
- Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College
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- Mineoka Tetsuro
- Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College
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- Yoshida Akihiro
- Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College
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- Ansai Toshihiro
- Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College
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- Takehara Tadamichi
- Division of Community Oral Health Science, Department of Health Promotion, Kyushu Dental College
Bibliographic Information
- Other Title
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- Relationship between endothelin-1 and interleukin-1β in inflamed periodontal tissues
Abstract
We recently demonstrated that endothelin-1 (ET-1) was strongly expressed in inflamed gingival tissues, but the biological role of ET-1 in gingival tissue remains unknown. This study focused on the relationship between ET-1 and interleukin-1β (IL-1β), an important cytokine during the periodontal inflammatory process. We determined the protein levels of ET-1 and IL-1β in gingival tissues from patients and examined whether ET-1 could regulate the expression of the IL-1β gene and protein in oral epithelial cells and fibroblasts in vitro. There was a significant correlation between the levels of ET-1 and IL-1β in 26 gingival tissues, as determined by ELISA. Following the confirmation of two specific ET-1 receptors (ETA and ETB receptors) on the cultured cells, the effects of ET-1 stimulation on IL-1β mRNA and protein expression were evaluated by RT-PCR and ELISA, respectively. The IL-1β mRNA and protein levels were enhanced by ET-1 stimulation in a dose-dependent manner, and the enhancement of IL-1β was inhibited by ETA or ETB receptor antagonists. These findings indicate that ET-1 is involved in the regulation of IL-1β expression in gingival tissues and suggest that ET-1 signaling to the cells may be a therapeutic target for treating IL-1β-dependent inflammatory responses.
Journal
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- Biomedical Research
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Biomedical Research 30 (6), 349-355, 2009
Biomedical Research Press
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Details 詳細情報について
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- CRID
- 1390001204903075584
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- NII Article ID
- 130004903774
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- ISSN
- 1880313X
- 03886107
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed