Different distribution of Cav3.2 and Cav3.1 transcripts encoding T-type Ca2+ channels in the embryonic heart of mice
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We investigated the distribution of T-type Ca<SUP>2+</SUP> channel mRNAs in the mouse embryonic heart. <I>Cav3.2</I>, but not <I>Cav3.1</I>, was expressed in the E8.5 embryonic heart along with cardiac progenitor markers (<I>Nkx2.5</I>, <I>Tbx5</I>, <I>Isl-1</I>) and contractile proteins (<I>alpha</I> and <I>beta MHC</I>). In the E10.5 heart, the distribution of <I>Cav3.1</I> mRNA was confirmed in the AV-canal and overlapped with that of <I>MinK</I> or <I>Tbx2</I>. <I>Cav3.2</I> mRNA was observed not only in the AV-canal but also in the outflow tract, along with <I>MinK</I> and <I>Isl-1</I>, indicating the expression of <I>Cav3.2</I> in the secondary heart field. Thus, <I>Cav3.2</I> may contribute to the development of the outflow tract from the secondary heart field in the embryonic heart, whereas <I>Cav3.1</I> may be involved in the development of the cardiac conduction-system together with <I>Cav3.2</I>.
- Biomedical Research
Biomedical Research 31(5), 301-305, 2010
Biomedical Research Press