Immunolocalization of DMP1 and sclerostin in the epiphyseal trabeculae and diaphyseal cortical bone of osteoprotegerin deficient mice

• Masuki Hideo

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University Department of Periodontology and Endodontology, Graduate School of Dental Medicine, Hokkaido University

• Li Minqi

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University

• Hasegawa Tomoka

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University

• Suzuki Reiko

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University

• Ying Guo

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University Department of Periodontology and Endodontology, Graduate School of Dental Medicine, Hokkaido University

• Zhusheng Liu

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University

• Oda Kimimitsu

  Division of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences

• Yamamoto Tsuneyuki

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University

• Kawanami Masamitsu

  Department of Periodontology and Endodontology, Graduate School of Dental Medicine, Hokkaido University

• Amizuka Norio

  Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University

書誌事項

タイトル別名

• Immunolocalization of DMP1 and sclerostin in the epiphyseal trabecule and diaphyseal cortical bone of osteoprotegerin deficient mice

抄録

In order to define the osteocytic function in accelerated bone remodeling, we examined the distribution of the osteocytic lacunar-canalicular system (OLCS) and osteocyte-secreting molecules-dentin matrix protein (DMP) 1 and sclerostin-in the epiphyses and cortical bones of osteoprotegerin deficient (OPG^-/-) mice. Silver impregnation visualized a well-arranged OLCS in the wild-type epiphyses and cortical bone, whereas OPG^-/- mice had an irregular OLCS in the epiphyses, but well-arranged canaliculi in the cortical bone. DMP1-positive osteocytes were evenly distributed throughout the wild-type epiphyses and cortical bone, as well as the OPG^-/- cortical bone. However, OPG^-/- epiphyses revealed weak DMP1-immunoreactivity. Thus, osteocytes appear to synthesize more DMP1 as the OLCS becomes regular. In contrast, sclerostin-immunoreactivity was significantly diminished in the OPG^-/- epiphyses and cortical bone. In OPG^-/- epiphyses and cortical bone, triple staining demonstrated few sclerostin-positive osteocytes in the periphery of a thick cell layer of alkaline phosphatase-positive osteoblasts and many tartrate resistant acid phosphatasepositive osteoclasts. Summarizing, the regular distribution of OLCS may affect DMP1 synthesis, while the cellular activities of osteoclasts and osteoblasts rather than the regularity of OLCS may ultimately influence sclerostin synthesis.

収録刊行物

• Biomedical Research

  Biomedical Research 31 (5), 307-318, 2010

  バイオメディカルリサーチプレス

キーワード

• General Biochemistry, Genetics and Molecular Biology
• General Medicine

詳細情報

• CRID: 1390282679878095616
• NII論文ID: 130004903810
• NII論文ID: 80021404167
• NII書誌ID: AA00110128
• DOI: 10.2220/biomedres.31.307
• ISSN: 1880313X
• ISSN: 03886107
• ISSN: http://id.crossref.org/issn/03886107
• HANDLE: 2115/72271
• Web Site: https://www.jstage.jst.go.jp/article/biomedres/31/5/31_5_307/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2011206155
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可