Morphological assessment of bone mineralization in tibial metaphyses of ascorbic acid-deficient ODS rats

• Hasegawa Tomoka

  Department of 1 Developmental Biology of Hard Tissue, Hokkaido University, Sapporo, Japan

• Li Minqi

  Department of 1 Developmental Biology of Hard Tissue, Hokkaido University, Sapporo, Japan

• Hara Kuniko

  Pharmacological Evaluation Section, Eisai Co., Ltd., Tokyo, Japan

• Sasaki Muneteru

  Department of 1 Developmental Biology of Hard Tissue, Hokkaido University, Sapporo, Japan

• Tabata Chihiro

  Department of 1 Developmental Biology of Hard Tissue, Hokkaido University, Sapporo, Japan

• Freitas Paulo Henrique Luiz de

  Department of Oral and Maxillofacial Surgery, Dr. Mário Gatti Municipal Hospital, Campinas, Brazil

• Hongo Hiromi

  Department of 1 Developmental Biology of Hard Tissue, Hokkaido University, Sapporo, Japan

• Suzuki Reiko

  Department of 1 Developmental Biology of Hard Tissue, Hokkaido University, Sapporo, Japan

• Kobayashi Masatoshi

  Pharmacological Evaluation Section, Eisai Co., Ltd., Tokyo, Japan

• Inoue Kiichiro

  Department of Oral Functional Anatomy, Hokkaido University, Sapporo, Japan

• Yamamoto Tsuneyuki

  Department of 1 Developmental Biology of Hard Tissue, Hokkaido University, Sapporo, Japan

• Oohata Noboru

  Department of Oral Rehabilitation, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan

• Oda Kimimitsu

  Division of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

• Akiyama Yasuhiro

  Pharmacological Evaluation Section, Eisai Co., Ltd., Tokyo, Japan

• Amizuka Norio

  Department of 1 Developmental Biology of Hard Tissue, Hokkaido University, Sapporo, Japan

抄録

Osteogenic disorder shionogi (ODS) rats carry a hereditary defect in ascorbic acid synthesis, mimicking human scurvy when fed with an ascorbic acid-deficient (aa-def) diet. As aa-def ODS rats were shown to feature disordered bone formation, we have examined the bone mineralization in this rat model. A fibrous tissue layer surrounding the trabeculae of tibial metaphyses was found in aa-def ODS rats, and this layer showed intense alkaline phosphatase activity and proliferating cell nuclear antigen-immunopositivity. Many osteoblasts detached from the bone surfaces and were characterized by round-shaped rough endoplasmic reticulum (rER), suggesting accumulation of malformed collagen inside the rER. Accordingly, fine, fragile fibrillar collagenous structures without evident striation were found in aa-def bones, which may result from misassembling of the triple helices of collagenous α-chains. Despite a marked reduction in bone formation, ascorbic acid deprivation seemed to have no effect on mineralization: while reduced in number, normal matrix vesicles and mineralized nodules could be seen in aa-def bones. Fine needle-like mineral crystals extended from these mineralized nodules, and were apparently bound to collagenous fibrillar structures. In summary, collagen mineralization seems unaffected by ascorbic acid deficiency in spite of the fine, fragile collagenous fibrils identified in the bones of our animal model.

収録刊行物

• Biomedical Research

  Biomedical Research 32 (4), 259-269, 2011

  バイオメディカルリサーチプレス

キーワード

• General Biochemistry, Genetics and Molecular Biology
• General Medicine

詳細情報

• CRID: 1390282679879142912
• NII論文ID: 130004903823
• NII書誌ID: AA00110128
• DOI: 10.2220/biomedres.32.259
• ISSN: 1880313X
• ISSN: 03886107
• ISSN: http://id.crossref.org/issn/03886107
• PubMed: 21878733
• Web Site: https://search.jamas.or.jp/link/ui/2012123103
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可