<b>Characterization of mesenchymal progenitor cells in the crown and root pulp of primary </b><b>teeth </b>
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- TORIUMI Taku
- Nihon University Graduate School of Dentistry
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- TAKAYAMA Naoya
- Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University
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- MURAKAMI Miyako
- Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo
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- SATO Momoko
- Department of Pediatric Dentistry, Nihon University School of Dentistry
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- YUGUCHI Maki
- Department of Anatomy, Nihon University School of Dentistry Dental Research Center, Nihon University School of Dentistry
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- YAMAZAKI Yosuke
- Department of Anatomy, Nihon University School of Dentistry Dental Research Center, Nihon University School of Dentistry
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- ETO Koji
- Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University
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- OTSU Makoto
- Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo
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- NAKAUCHI Hiromitsu
- Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo
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- SHIRAKAWA Tetsuo
- Department of Pediatric Dentistry, Nihon University School of Dentistry Dental Research Center, Nihon University School of Dentistry
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- ISOKAWA Keitaro
- Department of Anatomy, Nihon University School of Dentistry Dental Research Center, Nihon University School of Dentistry
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- HONDA Masaki J.
- Department of Anatomy, Nihon University School of Dentistry Dental Research Center, Nihon University School of Dentistry
Bibliographic Information
- Other Title
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- Characterization of mesenchymal progenitor cells in the crown and root pulp of primary teeth
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Abstract
The existence of progenitor/mesenchymal stem cells (MSCs) was demonstrated previously in human primary/deciduous teeth. In this study, we examined dental pulp cells from root portion (root cells) of primary teeth without discernible root resorption and compared them with pulp cells from the crown portion (crown cells). Root cells and crown cells were characterized and compared to each other based on progenitor/MSC characteristics and on their generation efficiency of induced pluripotent stem (iPS) cells. Root cells and crown cells included cells manifesting typical progenitor/MSC properties such as osteogenic and adipogenic differentiation potential and clonogenicity. Interestingly, root cells showed a higher expression level of embryonic stem cell marker, KLF4, than crown cells. Moreover, the number of colony-forming unit-fibroblast and cell proliferation rate were higher for root cells than crown cells, and the efficiency of generating iPS cells from root cells was approximately four times higher than that from crown cells. Taken together, these results suggest that root cells from primary teeth show the MSC-like properties and thus could be a potent alternative source for iPS cell generation and the subsequent transplantation therapy.
Journal
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- Biomedical Research
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Biomedical Research 36 (1), 31-45, 2015
Biomedical Research Press