Proteomic analysis of ethanol-induced embryotoxicity in cultured post-implantation rat embryos
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- Usami Makoto
- Division of Pharmacology, National Institute of Health Sciences
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- Mitsunaga Katsuyoshi
- School of Pharmaceutical Sciences, Toho University
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- Irie Tomohiko
- Division of Pharmacology, National Institute of Health Sciences
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- Miyajima Atsuko
- Division of Medical Devices, National Institute of Health Sciences
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- Doi Osamu
- Laboratory of Animal Reproduction, United Graduate School of Agricultural Science, Gifu University
Bibliographic Information
- Other Title
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- Toxicomics Report : Proteomic analysis of ethanol-induced embryotoxicity in cultured post-implantation rat embryos
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Abstract
Protein expression changes were examined in day 10.5 rat embryos cultured for 24 hr in the presence of ethanol by using two-dimensional electrophoresis and mass spectrometry. Exposure to ethanol resulted in quantitative changes in many embryonic protein spots (16 decreased and 28 increased) at in vitro embryotoxic concentrations (130 and 195 mM); most changes occurred in a concentration-dependent manner. For these protein spots, 17 proteins were identified, including protein disulfide isomerase A3, alpha-fetoprotein, phosphorylated cofilin-1, and serum albumin. From the gene ontology classification and pathway mapping of the identified proteins, it was found that ethanol affected several biological processes involving oxidative stress and retinoid metabolism.
Journal
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- The Journal of Toxicological Sciences
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The Journal of Toxicological Sciences 39 (2), 285-292, 2014
The Japanese Society of Toxicology
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Keywords
Details 詳細情報について
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- CRID
- 1390282679882440320
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- NII Article ID
- 130004903990
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- NII Book ID
- AN00002808
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- COI
- 1:STN:280:DC%2BC2crltlCgug%3D%3D
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- ISSN
- 18803989
- 03881350
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- NDL BIB ID
- 025456426
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- PubMed
- 24646710
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed