Effect of maternal exposure to carbon black nanoparticle during early gestation on the splenic phenotype of neonatal mouse

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  • Shimizu Ryuhei
    Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science
  • Umezawa Masakazu
    Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science The Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Tokyo University of Science
  • Okamoto Saki
    Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science
  • Onoda Atsuto
    Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science
  • Uchiyama Mariko
    Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science
  • Tachibana Ken
    The Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Tokyo University of Science
  • Watanabe Shiho
    Division of Immunobiology, Research Institute for Biomedical Sciences, Tokyo University of Science
  • Ogawa Shuhei
    The Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Tokyo University of Science Division of Immunobiology, Research Institute for Biomedical Sciences, Tokyo University of Science
  • Abe Ryo
    Division of Immunobiology, Research Institute for Biomedical Sciences, Tokyo University of Science
  • Takeda Ken
    Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science The Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Tokyo University of Science

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Abstract

Maternal exposure to environmental factors is implicated as a major factor in the development of the immune system in newborns. Newborns are more susceptible to microbial infection because their immune system is immature. Development of lymphocytes reflects an innate program of lymphocyte proliferation. The aim of this study was to investigate the effects of maternal exposure to carbon black nanoparticle (CB-NP) during early gestation on the development of lymphoid tissues in infantile mice. Pregnant ICR mice were treated with a suspension of CB-NP (95 μg kg-1 time-1) by intranasal instillation on gestational day 5 and 9. Spleen tissues were collected from offspring mice at 1, 3, 5, and 14 days postpartum. Splenocyte phenotypes were examined by investigating the pattern of surface molecules using flow cytometry. Gene expression in the spleen was examined by quantitative RT-PCR. CD3+ (T), CD4+ and CD8+ cells were decreased in the spleen of 1-5-day-old offspring in the treated group. Expression level of Il15 was significantly increased in the spleen of newborn male offspring, and Ccr7 and Ccl19 were increased in the spleen of female offspring in the CB-NP group. Splenic mRNA change profiles by CBNP were similar between male and female offspring. This article concluded that exposure of pregnant mothers to CB-NP partially suppressed the development of the immune system of offspring mice. The decrease in splenic T cells in the treated group recovered at 14 days after birth. This is the first report of developmental effect of nanoparticle on the lymphatic phenotype.

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