Spontaneous seizures in a rat models of multiple prenatal lesioning
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- Kamada Takashi
- Epilepsy Center, Fukuoka Sanno Hospital
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- Takase Kei-ichiro
- Department of Neurology, Iizuka Hospital
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- Shigeto Hiroshi
- Department of Neurology, Neurological Institute, Graduate School of Medical Sciences, Kyushu University
Bibliographic Information
- Other Title
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- 自発てんかん発作を有した皮質異形成ラットモデル
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Abstract
Focal cortical dysplasia (FCD) is an important cause of intractable epilepsy. Previous rat studies have utilized freeze lesioning of neonatal animals to model FCD; however, such models are unable to demonstrate spontaneous seizures without seizure-provoking events. Therefore, we created an animal model with multiple FCD, produced during embryonic development, and observed whether spontaneous seizures occurred. Furthermore, we examined the relationship between FCD and epileptogenesis using immunohistochemistry. At 18 days postconception, a frozen metal probe was placed bilaterally on the scalps of Sprague-Dawley rat embryos through the uterus wall to produce multiple FCD. Eleven of 16 rats showed spontaneous seizures arising in the hippocampus from postnatal day47. Movement cessation followed by sniffing and mastication, culminating in wet-dog shaking, was seen during the hippocampal EEG discharges. Alterations in the levels of glutamatergic and GABA-ergic receptors were investigated during growth. We created an animal model showing spontaneous seizures without a provoking event except for the existence of cortical dysplasia, and without a genetic or general systematic cause like MAM injection or irradiation. The seizures resembled human temporal lobe epilepsy both clinically and on EEG. This model should enable better clarification of the mechanisms underlying the development of human epilepsy.
Journal
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- Rinsho Shinkeigaku
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Rinsho Shinkeigaku 54 (12), 1132-1135, 2014
Societas Neurologica Japonica