Renal Tubular Secretion of Varenicline by Multidrug and Toxin Extrusion (MATE) Transporters
-
- KAJIWARA Moto
- Department of Pharmacy, Kyoto University Hospital
-
- MASUDA Satohiro
- Department of Pharmacy, Kyoto University Hospital
-
- WATANABE Shingo
- Department of Pharmacy, Kyoto University Hospital
-
- TERADA Tomohiro
- Department of Pharmacy, Kyoto University Hospital
-
- KATSURA Toshiya
- Department of Pharmacy, Kyoto University Hospital
-
- INUI Ken-ichi
- Department of Pharmacy, Kyoto University Hospital
この論文をさがす
抄録
Multidrug and toxin extrusion (MATE) 1 and MATE2-K, H+/organic cation antiporters, are located at the brush-border membrane of renal proximal tubules. The present study aimed to clarify the role of MATE transporters in tubular secretion of varenicline. Varenicline at a dose of 5 mg/kg was administered to wild-type and Mate1-knockout mice via the jugular vein, and its uptake was measured by high-performance liquid chromatography. The renal secretory clearance of and systemic exposure to varenicline were significantly decreased (54.6%, p < 0.05) and increased (116%, p < 0.05) respectively, by the genetic disruption of Mate1 in mice. Uptake of varenicline and [14C]tetraethylammonium (TEA) was examined in HEK293 cells transiently expressing the human (h) MATE1, hMATE2-K, mouse (m) MATE1, and hOCT2 basolateral organic cation transporter. [14C]TEA uptake in HEK293 cells expressing MATE transporters and hOCT2 was decreased in the presence of varenicline. The calculated IC50 values for hMATE1, hMATE2-K, mMATE1, and hOCT2 were 62.2 ± 6.5, 122.3 ± 67.6, 255.0 ± 37.9, and 1,003.9 ± 135.8 (µM; mean ± S.E. for three separate experiments), respectively. Varenicline uptake was significantly increased in HEK293 cells expressing mMATE1, hMATE1, or hMATE2-K cDNA as well as hOCT2 compared to empty vector-transfected cells. In conclusion, renal MATE transporters were found to be responsible for renal tubular secretion of varenicline.<br>
収録刊行物
-
- Drug Metabolism and Pharmacokinetics
-
Drug Metabolism and Pharmacokinetics 27 (6), 563-569, 2012
日本薬物動態学会