Observation of a 3D Network Nano-Structure of Carbon Nanotubes Scaffold for Cultivation

  • Abe Shigeaki
    Graduate School of Dental Medicine, Hokkaido University, Japan
  • Ishikawa Kosuke
    Graduate School of Dental Medicine, Hokkaido University, Japan
  • Hyono Atsushi
    Division of Materials Science and Engineering, Faculty of Engineering, Hokkaido University, Japan
  • Kobayashi Hirohisa
    Division of Biofunctional Polymers, Graduate School of Engineering, Hokkaido University, Japan
  • Kiba Takayuki
    Division of Biofunctional Polymers, Graduate School of Engineering, Hokkaido University, Japan
  • Akasaka Tsukasa
    Graduate School of Dental Medicine, Hokkaido University, Japan
  • Uo Motohiro
    Graduate School of Dental Medicine, Hokkaido University, Japan
  • Yawaka Yasutaka
    Graduate School of Dental Medicine, Hokkaido University, Japan
  • Sato Shin-Ichiro
    Division of Biofunctional Polymers, Graduate School of Engineering, Hokkaido University, Japan
  • Yonezawa Tetsu
    Division of Materials Science and Engineering, Faculty of Engineering, Hokkaido University, Japan
  • Watari Fumio
    Graduate School of Dental Medicine, Hokkaido University, Japan

Abstract

Development of scaffolds consisting of micro-/nano-sized materials have attracted a great deal of attention for their potential use in tissue engineering tools. We prepared a cell culture scaffold of carbon nanotubes, which is a typical bio-inert nanomaterial, and then investigated the surface morphology and properties. A three-dimensional nano-level network structure was observed using a scanning electron microscope and an atomic force microscope. The scaffold also exhibited excellent protein absorption. In order to apply the obtained scaffold to the cultivation of osteoblast cells, the cytocompatibility was comparable to that of a conventional cell culture dish. [DOI: 10.1380/ejssnt.2011.80]

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