Screening of High-permissive Silkworm Strains for Efficient Recombinant Protein Production in <i>Autographa californica</i> Nuclear Polyhedrosis Virus (AcNPV)

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Author(s)

    • Lee Jae Man Man Lee Jae
    • Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
    • Takahashi Masateru
    • Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
    • Kawakami Naoya
    • Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
    • Mitsunobu Hitoshi
    • Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
    • Banno Yutaka
    • Laboratory of Insect Genetic Resources, Faculty of Agriculture, Kyushu University
    • Koga Katsumi
    • Department of Biological Substances and Life Science, Kyushu Kyoritsu University
    • Uchino Keiro
    • Transgenic Silkworm Research Center, National Institute of Agrobiological Sciences
    • Kawaguchi Yutaka
    • Laboratory of Silkworm Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences

Abstract

The baculovirus expression system (BES) using <i>Autographa californica</i> nuclear polyhedrosis virus (AcNPV) has been extensively utilized for the high-level expression of sufficient quantities of recombinant proteins in a broad taxonomic range of insect cell lines and insect larvae. In the case of the silkworm, <i>Bombyx mori</i>, however, the BES using AcNPV tends to be much less exploited because AcNPV is believed to be inefficient in the replication of <i>B. mori</i> and cell lines derived from it. In this study, we have searched for high-permissive silkworm strains with higher production levels of a recombinant protein, by screening the susceptibility of 163 silkworm strains to the recombinant AcNPV, which expresses firefly luciferase. Based on their relative luciferase expression levels in larval hemocytes, the silkworm strains tested were divided into 3 groups: 5 high-permissive strains, 74 middle-permissive strains, and 84 low-permissive strains. Among the 5 high-permissive strains (c11, d17, f10, f38, and l312), d17 was the most productive, showing the luciferase activity of 3.5×10<sup>4</sup>±1,764 relative light units (RLU) per microgram of cell proteins. This remarkable susceptibility indicated that the silkworm d17 strain is very useful for large-scale protein production by the BES using AcNPV.<br>

Journal

  • Journal of Insect Biotechnology and Sericology

    Journal of Insect Biotechnology and Sericology 76(2), 2_101-2_105, 2007

    The Japanese Society of Sericultural Science

Cited by:  1

Codes

  • NII Article ID (NAID)
    130004935558
  • NII NACSIS-CAT ID (NCID)
    AA11558849
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    1346-8073
  • NDL Article ID
    8781267
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産)
  • NDL Call No.
    Z54-J696
  • Data Source
    CJPref  NDL  J-STAGE 
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