Time-Dependent Changes in the Genetic Diversity and Structure of the Onagadori Breed of Chickens Based on Microsatellite DNA Polymorphisms

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Author(s)

    • Oka Takao Oka Takao
    • Graduate School of Biosphere Science, Hiroshima University, Japan|Japanese Avian Bioresource Project Research Center, Hiroshima University, Japan
    • Tadano Ryo
    • Graduate School of Biosphere Science, Hiroshima University, Japan|Faculty of Applied Biological Science, Gifu University, Japan
    • Goto Tatsuhiko
    • Graduate School of Biosphere Science, Hiroshima University, Japan|Japanese Avian Bioresource Project Research Center, Hiroshima University, Japan|Collage of Agriculture, Ibaraki University, Japan
    • Shiraishi Jun-ichi
    • Graduate School of Biosphere Science, Hiroshima University, Japan|Japanese Avian Bioresource Project Research Center, Hiroshima University, Japan|Department of Animal Science, Nippon Veterinary and Life Science University, Japan
    • A.M. Osman Sayed
    • Graduate School of Biosphere Science, Hiroshima University, Japan|Department of Genetics, Faculty of Agriculture, Minia University, Egypt
    • Nagasaka Naohiro
    • Graduate School of Biosphere Science, Hiroshima University, Japan|Japanese Avian Bioresource Project Research Center, Hiroshima University, Japan|Kochi Prefectural Livestock Experimental Station, Japan
    • Bungo Takashi
    • Graduate School of Biosphere Science, Hiroshima University, Japan|Japanese Avian Bioresource Project Research Center, Hiroshima University, Japan
    • Maeda Teruo
    • Graduate School of Biosphere Science, Hiroshima University, Japan|Japanese Avian Bioresource Project Research Center, Hiroshima University, Japan
    • Nishibori Masahide
    • Graduate School of Biosphere Science, Hiroshima University, Japan|Japanese Avian Bioresource Project Research Center, Hiroshima University, Japan

Abstract

To conserve the Tosa-no-Onagadori (briefly Onagadori), one of the native Japanese chicken breeds designated as a Special National Natural Treasure of Japan, time-dependent changes in genetic diversity and structure were analyzed based on 20 microsatellites. From three varieties (black-breasted white, black-breasted red, and white) of the Onagadori reared in Nankoku City, Kochi Prefecture, Japan, blood samples were collected in 1999, 2005, and 2009-2010. Significant genetic differentiation was recognized among breeders and among varieties in the whole Onagadori population, but it was not observed between years. Focusing on breeders, some populations showed decrease in allelic richness (AR) and expected heterozygosity (<i>H</i><sub>E</sub>) with advancing years. Focusing on varieties, AR of the black-breasted red variety showed significant loss from 1999 to 2010, resulting in the significant loss of AR in the whole Onagadori population. In contrast, the AR and <i>H</i><sub>E</sub> of the black-breasted white and white varieties were constant across sampling years. The <i>D</i><sub>A</sub> genetic distance among populations decreased with sampling years, and genetic homogeneity increased in the whole Onagadori population. Structure analysis supported K=2, dividing the genetic constitution into Onagadori-specific one and the rest. In the black-breasted white variety, the birds that have Onagadori-specific genetic composition increased with time. On the contrary, in the black-breasted red and white varieties surveyed in 2009-2010, there was no bird that showed Onagadori-specific genetic background of more than 75%. To maintain the Onagadori in Nankoku City for a long term, regular monitoring of genetic diversity and systematic reproduction using excellent black-breasted white birds that have the Onagadori-specific genetic composition should be continued.

Journal

  • The Journal of Poultry Science

    The Journal of Poultry Science 51(3), 262-269, 2014

    Japan Poultry Science Association

Codes

  • NII Article ID (NAID)
    130004938579
  • NII NACSIS-CAT ID (NCID)
    AA11564513
  • Text Lang
    ENG
  • ISSN
    1346-7395
  • NDL Article ID
    025599174
  • NDL Call No.
    Z54-J694
  • Data Source
    NDL  J-STAGE 
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