β1-Integrin-Dependent Macrophage Migration is Regulated by MAP Kinase

DOI
  • SAKATA Takeichi
    Graduate School of Dentistry (Oral and Maxillofacial Surgery), Osaka Dental University
  • GODA Seiji
    Department of Biochemistry, Osaka Dental University
  • OGAWA Yumiko
    First Department of Oral and Maxillofacial Surgery, Osaka Dental University
  • IKEO Takashi
    Department of Biochemistry, Osaka Dental University
  • MORITA Shosuke
    First Department of Oral and Maxillofacial Surgery, Osaka Dental University

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Chemokines regulate the homeostatic trafficking of lymphocytes and lymphocyte influx to sites of injury and inflammation. Here, we investigate the role of CXCL12 on the migration and signaling of RAW264 cells. We find that CXCL12-induced migration is enhanced in a dose-dependent manner, and CXCL12 enhances phosphorylation of ERK1/2 and AKT. Furthermore, this CXCL12-stimulated migration and phosphorylation is inhibited in the presence of the MEK1/2 inhibitor U0126, and the PI 3-K inhibitors, Wortmannin and LY294002. However, MEK1/2 inhibitor does not affect phosphorylation of the PI 3-K enzyme AKT, and PI 3-K inhibitors do not affect the phosphorylation of ERK1/2. This suggests that stimulation of ERK1/2 and AKT by CXCL12 occurs via an independent signaling pathway, which enhances migration of RAW264 cells.

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