Dentin Regeneration by the Differentiated Human Bone Marrow Mesenchymal Stem Cells

• IZAWA Shunji

  Department of Operative Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

• YAMAJI Kozo

  Department of Operative Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

• HOSHIKA Tomohiro

  Department of Operative Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

• NAKATA Takashi

  Department of Operative Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

• SHINNO Yasuo

  Department of Operative Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

• NISHITANI Yoshihiro

  Department of Operative Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

• YOSHIYAMA Masahiro

  Department of Operative Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

Bibliographic Information

Other Title

• ヒト骨髄間葉系幹細胞を用いた象牙芽細胞分化と象牙質再生

Abstract

Dentin regeneration to the lost dentin is the supreme therapy to keep functionality, aesthetics and so forth in operative dentistry. In 2006, we demonstrated the human bone marrow mesenchymal stem cells (hMSCs) differentiated into odontoblast by the stimulation of Emdogain Gel and TNF-α set. In 2008, we tried to demonstrate odontoblast that differentiated from hMSCs generate dentin under the artificially synthesized appropriate scaffold and molecular signal in vitro. As a result, the differentiated cell tissue tested positive for osteocalcin by fluorescence antibody method and enzyme antibody technique and positive for calcium compounds with the alizarin red S staining. In addition, we detected the dentin-like tissue outside the cell walls by phase contrast microscopy. These results suggested that hMSCs differentiate into odontoblast and generate dentin using Emdogain Gel and TNF-α set and peptide hydrogel.

Journal

• Journal of the Japanese Association of Regenerative Dentistry

  Journal of the Japanese Association of Regenerative Dentistry 7 (1), 47-55, 2009

  Japanese Association of Regenerative Dentistry

Keywords

• human mesenchymal stem cells
• dentin regeneration
• Emdogain Gel
• TNF-α
• peptide hydrogel

Details

• CRID: 1390282680204086656
• NII Article ID: 130004941457
• DOI: 10.11223/jard.7.47
• ISSN: 18800815
• ISSN: 13489615
• Web Site: https://search.jamas.or.jp/link/ui/2010108645
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed