Three-dimensional imaging of growing thrombus in vivo

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Author(s)

    • Morikawa Takayuki
    • Department of Biochemistry and Integrative Medical Biology, Keio University School of Medicine
    • Kajimura Mayumi
    • Department of Biochemistry and Integrative Medical Biology, Keio University School of Medicine
    • Ichikawa Mio
    • Department of Biochemistry and Integrative Medical Biology, Keio University School of Medicine
    • Suematsu Makoto
    • Department of Biochemistry and Integrative Medical Biology, Keio University School of Medicine

Abstract

Although several methods have been developed to investigate thrombus or hemostatic plug formation in microvessels in vivo , our knowledge on platelet behavior during such a process is still limited. We, therefore, developed new instrumentation which enables us to induce interactions of individual platelets and to detect their behavior with endothelium in and around the growing thrombus. To evoke a platelet reaction, focal hemorrhage was induced with a nitrogen-dye laser focused through the microscope optics using mesenteric microcirculation of the rat. Platelet deposition at the site of injury was then observed during thrombus formation in real-time. The system was able to provide images with high spatial resolution at video-frame rate that could be used to reconstruct three-dimensional model of a thrombus. (Abstract movie) [MVRC 2 (1): 8-12,2008]

Journal

  • Microvascular Reviews and Communications

    Microvascular Reviews and Communications 2(1), 8-12, 2008

    Japanese Society for Microcirculation

Codes

  • NII Article ID (NAID)
    130004942918
  • Text Lang
    ENG
  • ISSN
    2188-1707
  • Data Source
    J-STAGE 
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