Discovering the true identity and function of mesenchymal stem cells
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Mesenchymal stem cells (MSCs) are currently defined as cells that undergo sustained <I>in vitro</I> growth and can give rise to multiple mesenchymal lineages. Traditional MSCs isolation methods require prolonged <I>in vitro</I> culture on plastic plates, which reduces their differentiation potential and proliferative ability. Furthermore, this process alters MSCs-phenotype, making it difficult to identify specific MSCs-markers that could be used for their <I>in vivo</I> localization and prospective isolation. These limitations have hindered investigations into the biology and function of MSCs. This review article focuses on recent developments in the MSC-research field including the identification of novel surface markers for the prospective isolation of both murine and human MSCs. Prospectively isolated MSCs are more proliferative than MSCs prepared by conventional plastic adherence, provide a better substrate for studying MSCs biology and have more potential for regenerative therapy.
- Inflammation and Regeneration
Inflammation and Regeneration 32(4), 146-151, 2012
The Japanese Society of Inflammation and Regeneration