Protective effect of CeO2 nanoparticles on photo-induced oxidative damage of DNA Protective effect of CeO<sub>2</sub> nanoparticles on photo-induced oxidative damage of DNA
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The present paper demonstrates that inorganic ceria (CeO<sub>2</sub>) nanoparticles effectively inhibit anomalous oxidative damage of DNA induced by ultraviolet (UV) light irradiation. To utilize the CeO<sub>2</sub> nanoparticles for the protection test of DNA, a colloidal solution of monodispersed and crystallized CeO<sub>2</sub> nanoparticles is synthesized through a photochemical reaction of Ce(NO<sub>3</sub>)<sub>3</sub> solution, followed by dialysis to remove unreacted electrolytes. Subsequently, the UV light induced oxidative damage of DNA in the presence or absence of CeO<sub>2</sub> nanoparticles is evaluated by a quantitative analysis of 8-hydroxy-2′-deoxyguanosine (8-OHdG) which is an oxidation product of guanine in base sequences. The 8-OHdG concentration in DNA is increased by the exposure to UV light. On the other hand, the co-presence of CeO<sub>2</sub> nanoparticles diminish the formation rate of 8-OHdG. Such favorable effect of CeO<sub>2</sub> nanoparticles is due to an excellent annihilation activity of reactive oxygen species (ROS) accounting for the DNA oxidation as well as a UV light absorption ability based on semiconducting nature.
- Journal of the Ceramic Society of Japan
Journal of the Ceramic Society of Japan 122(1422), 141-145, 2014
The Ceramic Society of Japan