書誌事項
- タイトル別名
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- Conversion of RFLP Markers for the Selection of Pollination-Constant and Non-Astringent Type Persimmons (Diospyros kaki Thunb.) into PCR-Based Markers
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抄録
Persimmon (Diospyros kaki Thunb.) cultivars are classified into 4 types depending on the relationship between astringency of the mature fruit and the effect of seeds on the loss of astringency, and only pollination-constant and non-astringent (PCNA)-type persimmons stably lose fruit astringency as a part of fruit development. This is a recessive trait, regarded to be controlled by a single locus, namely, the AST locus, which has a polysomic nature. Thus far, we have identified 2 restriction fragment length polymorphism (RFLP) markers, namely, A1 and A2, each of which is separately linked to a different AST allele, and proved that the RFLP markers were useful for selecting PCNA-type persimmons. This study was conducted to convert the RFLP markers into polymerase chain reaction (PCR)-based markers. We isolated and characterized genomic regions corresponding to each RFLP marker by inverse PCR. Two primer pairs, E4/E9r and E4/A2r, were designed to generate 2 sequence characterized amplified region (SCAR) markers, i.e., PCR-A1 and PCR-A2, respectively. The PCR-A1 and PCR-A2 markers cosegregated with the A1 and A2 markers, respectively, in ‘Nishimura-wase’-derived progenies. Although the primer pair E4/A2r did not produce the PCR-A2 marker in the FU-275, which is a progeny derived from ‘Aizumishirazu’, all non-PCNA-type offspring but no PCNA-type offspring showed the PCR-A1 marker using the primer pair E4/E9r. Thus, it was revealed that the SCAR markers were useful for selecting PCNA-type offspring in these progenies. On the other hand, disruption of the relationship between the markers and the AST locus was observed in the KU-325, derived from ‘Kurokuma’, indicating that the selection of PCNA-type offspring using PCR-based markers is not effective for progeny derived from ‘Kurokuma’. Herein, we discuss the possibility of applying PCR-based markers to genetic studies.<br>
収録刊行物
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 78 (1), 68-73, 2009
一般社団法人 園芸学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390282680266826752
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- NII論文ID
- 110007044033
- 130004951578
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- NII書誌ID
- AA12177046
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- ISSN
- 1882336X
- 18823351
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- NDL書誌ID
- 9768731
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可