Conversion of RFLP Markers for the Selection of Pollination-Constant and Non-Astringent Type Persimmons (Diospyros kaki Thunb.) into PCR-Based Markers
-
- Kanzaki Shinya
- Labratory of Horticultural Science, Faculty of Agriculture, Kinki University
-
- Yamada Masahiko
- Grape and Persimmon Research Station, National Institute of Fruit Tree Science
-
- Sato Akihiko
- Grape and Persimmon Research Station, National Institute of Fruit Tree Science
-
- Mitani Nobuhito
- Grape and Persimmon Research Station, National Institute of Fruit Tree Science
-
- Ustunomiya Naoki
- Labratory of Horticultural Science, Faculty of Agriculture, Kinki University
-
- Yonemori Keizo
- Laboratory of Pomology, Graduate School of Agriculture, Kyoto University
Bibliographic Information
- Other Title
-
- 完全甘ガキ選抜に有効な RFLP マーカーの PCR マーカーへの変換
Search this article
Abstract
Persimmon (Diospyros kaki Thunb.) cultivars are classified into 4 types depending on the relationship between astringency of the mature fruit and the effect of seeds on the loss of astringency, and only pollination-constant and non-astringent (PCNA)-type persimmons stably lose fruit astringency as a part of fruit development. This is a recessive trait, regarded to be controlled by a single locus, namely, the AST locus, which has a polysomic nature. Thus far, we have identified 2 restriction fragment length polymorphism (RFLP) markers, namely, A1 and A2, each of which is separately linked to a different AST allele, and proved that the RFLP markers were useful for selecting PCNA-type persimmons. This study was conducted to convert the RFLP markers into polymerase chain reaction (PCR)-based markers. We isolated and characterized genomic regions corresponding to each RFLP marker by inverse PCR. Two primer pairs, E4/E9r and E4/A2r, were designed to generate 2 sequence characterized amplified region (SCAR) markers, i.e., PCR-A1 and PCR-A2, respectively. The PCR-A1 and PCR-A2 markers cosegregated with the A1 and A2 markers, respectively, in ‘Nishimura-wase’-derived progenies. Although the primer pair E4/A2r did not produce the PCR-A2 marker in the FU-275, which is a progeny derived from ‘Aizumishirazu’, all non-PCNA-type offspring but no PCNA-type offspring showed the PCR-A1 marker using the primer pair E4/E9r. Thus, it was revealed that the SCAR markers were useful for selecting PCNA-type offspring in these progenies. On the other hand, disruption of the relationship between the markers and the AST locus was observed in the KU-325, derived from ‘Kurokuma’, indicating that the selection of PCNA-type offspring using PCR-based markers is not effective for progeny derived from ‘Kurokuma’. Herein, we discuss the possibility of applying PCR-based markers to genetic studies.<br>
Journal
-
- Journal of the Japanese Society for Horticultural Science
-
Journal of the Japanese Society for Horticultural Science 78 (1), 68-73, 2009
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282680266826752
-
- NII Article ID
- 110007044033
- 130004951578
-
- NII Book ID
- AA12177046
-
- ISSN
- 1882336X
- 18823351
-
- NDL BIB ID
- 9768731
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
-
- Abstract License Flag
- Disallowed