<b>Effects of High Ambient Temperature on Plasma 3-methylhistidine Concentration and Expression of Muscle Calpain and Calpastatin mRNA in High-yielding Lactating Holstein Co</b><b>ws </b>

DOI Web Site
  • KAMIYA Mitusru
    National Agricultural Research Center for Kyushu Okinawa Region, NARO, Japan
  • MUROYA Susumu
    National Institute of Livestock and Grassland Science, NARO, Japan
  • KAMIYA Yuko
    National Agricultural Research Center for Kyushu Okinawa Region, NARO, Japan
  • KAMADA Toshihiko
    Tokyo University of Agriculture and Technology, Japan Present address: Yamazaki Gakuen University, Japan
  • TANAKA Masahito
    National Agricultural Research Center for Kyushu Okinawa Region, NARO, Japan

Bibliographic Information

Other Title
  • Effects of High Ambient Temperature on Plasma 3-methylhistidine Concentration and Expression of Muscle Calpain and Calpastatin mRNA in High-yielding Lactating Holstein Cows

Search this article

Abstract

<tt>The effect of high ambient temperature on myofibrillar proteolysis in high-yielding lactating cows was investigated. In a 2 x 2 crossover design, four multiparous lactating Holstein cows were maintained in a chamber under conditions of constant moderate ambient temperature (MT) or high ambient temperature (HT). Dry matter intake (P < 0.10) and milk yield (P < 0.10), and milk protein yield (P < 0.05) were lower in HT than MT. Urinary 3-methylhistidine excretion were 1.87 mmol/day in MT and 1.92 mmol/day in HT, but no statistically significant difference was found. Plasma 3-methylhistidine concentrations were higher (P < 0.05) in HT (6.9 nmol/ml) than MT (4.1 nmol/ml), indicating that high ambient temperature increased the myofibrillar proteolysis. The expression of muscle calpain SS (small subunit (28kDa)), -1, and -3, and calpastatin mRNA in HT were increased 1.4, 1.1, 1.6, and 1.4-fold of MT, respectively, but no statistically significant difference was found. Meanwhile, the correlation between the level of plasma 3-methylhistidine and the level of muscle calpain 3 mRNA was significant (P < 0.05) and its correlation coefficient was the highest in calpain family. In conclusion, high ambient temperature affected the plasma index of myofibrillar proteolysis related to the expression of muscle calpain 3 </tt><tt>and calpastatin mRNA in high-yielding lactating dairy cows. </tt>

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top