市販ドッグフードの脂質に対するα-トコフェロールの抗酸化効果

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  • Antioxidant effect of α-tocopherol on fat in commercial dog foods
  • シハン ドッグ フード ノ シシツ ニ タイスル アルファ トコフェロール ノ コウサンカ コウカ

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We added α-tocopherol to 5 different commercial dog foods after their bags were opened to see how their fat changes in terms of acid value, peroxide value, and iodine value for 30 days. The total amount of Vitamin E was also measured. The fat contents of the dog foods were 6% (in 2 of them),10%,15% and 18%.<BR>1. The acid value of the 5 dog foods ranged from 4.7 mg/g and 7.3 mg/g immediately after their bags were opened. These values were not toxic to dogs. The acid values in those dog foods changed little with time, so α-tocopherol did not show any antioxidant effect. The acid values of the dog foods during storage were within the safe range for dogs.<BR>2. Judging by the changes in the value of peroxide value with time, the amounts of α-tocopherol that we used in our experiment did not seem to be enough to keep the value of peroxide value stable.<BR>3. The iodine values of all the 5 dog foods used in this experiment tended to decline with time, and their values were less than 90. So, we judged that the fat in the dog foods is a nondrying oil. The addition of α-tocopherol resulted in little change in the iodine value.<BR>4. For the analysis of Vitamin E with HPLC, we tried to cut the extraction time and save the amount of reagent by reducing the number of washing when washing the water layer with petroleum ether after the saponification of the reagent. We found that the extract after two washing still contained Vitamin E which is about 10% of the amount found in the extract after the first washing. So we chose to repeat the washing process twice for our analysis. In the total amount of Vitamin E, α-tocopherol was 97% of the total in the control and 99% in the dog foods with addedatocopherol. The amounts of β-tocopherol and γ-tocopherol were 0.8-1.9%. The amount of α-tocopherol in the dog foods tended to decline with time due to fat rancidity. The detected amounts of β-and Y-tocopherol were small, indicating that there was no anti-oxidant effect of α-tocopherol.

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