Shape Transformation of Adsorbed Vesicles on Oxide Surfaces: Effect of Substrate Material and Photo-Irradiation

• Tero Ryugo

  Division of Biomolecular Sensing, Institute for Molecular Science

• Ujihara Toru

  Department of Crystalline Materials Science, Graduate School of Engineering, Nagoya University

• Urisu Tsuneo

  Division of Biomolecular Sensing, Institute for Molecular Science

Abstract

Shape transformation of phospholipid vesicles on oxide surfaces was investigated by a fluorescence microscope. The transformation of spherical vesicles to a planar lipid bilayer membrane spontaneously proceeded on mica and glass, while the intact vesicular layer formed on TiO₂. Interaction energy between the substrate and the bilayer, which was evaluated using the rigorously calculated Hamaker constant, was ～10 times larger on TiO₂ than on mica and SiO₂. The results seems inconsistent with the conventionally proposed adhesion induced tension model, in which stronger adsorption leads to easier planar membrane formation from vesicles, thus indicate that the shape transformation from vesicles to a planar membrane is dominated by the kinetic processes and the dynamics of the vesicles, rather than the adsorption state of individual vesicle. Area-selective SPB formation of adsorbed vesicles was induced by the irradiation of strong excitation light, which was assisted by the photo-induced expansion of SPB containing dye-labeled lipid molecules.

Journal

• Transactions of the Materials Research Society of Japan

  Transactions of the Materials Research Society of Japan 34 (2), 183-188, 2009

  The Materials Research Society of Japan

Keywords

• lipid bilayer membrane
• liposome
• mica
• silicon oxide
• titanium oxide
• fluorescence microscope

Details

• CRID: 1390282680489786624
• NII Article ID: 130005003953
• DOI: 10.14723/tmrsj.34.183
• COI: 1:CAS:528:DC%2BD1MXhtVOms7zM
• ISSN: 21881650
• ISSN: 13823469
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed