動的マイクロ力学刺激を用いたヒト骨芽細胞の操作・培養コントロール  [in Japanese] Manipulation and control of cultured human osteoblast based on micro dynamic stimulation  [in Japanese]

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Abstract

本研究では,細胞の力学特性を測定検出するために細胞膜に着目し,細胞膜のやわらかさを測定可能なセンサー機能と微小な細胞に動的力学刺激を与えることができるアクチュエータ機能を持つ圧電マイクロデバイスを開発した.本デバイスを用いて,ヒト骨芽細胞のストレスファイバー量と細胞膜のやわらかさの関係性を評価する実験を行った.その結果,両者に一定の相関がみられた.また,ストレスファイバーの再構築過程において,細胞は外部の力学刺激の影響を受けやすいことが明らかになった.

In this study, a new sensor system is developed to detect compliance of cultured cells by using dynamic response of a piezoelectric vibrator. The sensor was integrally controlled by a micromanipulator system, while dimensions and morphologies of the cells were measured by an inverted phase contrast microscope system. Experimental studies have been carried out using normal human osteoblast. The studies have shown the sensor's capability to detect changes of mechanical properties of the cultured cells. Also, the method has shown the relationship between compliance and repolymerization time of the stress fiber.

Journal

  • Proceedings of the Japan Joint Automatic Control Conference

    Proceedings of the Japan Joint Automatic Control Conference 51(0), 104-104, 2008

    The Japan Joint Automatic Control Conference

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