Development of DNA-Containing Nano Scale Vesicle by Reverse Micelles

• Morita Mitsuhiro

  Department of Chemical Engineering, Kyushu University, Japan

• Ono Tsutomu

  Department of Chemical Engineering, Kyushu University, Japan

• Sakai Shinji

  Department of Chemical Engineering, Kyushu University, Japan

• Ijima Hiroyuki

  Department of Chemical Engineering, Kyushu University, Japan

• Kawakami Koei

  Department of Chemical Engineering, Kyushu University, Japan

抄録

We report a novel method to prepare nano scale vesicles with high encapsulation efficiency of target nucleotides. First, DNA was extracted from an aqueous solution into a cationic reverse micellar solution consisting of dimethyldistearylammonium bromide (2C18AB). Secondly, small amount of the reverse micellar solution containing DNA was dropped into an aqueous surfactant solution to form the outer-layer of vesicles. We mainly employed polyoxyethylene (20) sorbitan monolaurate (Tween 20) or cetyltrimethylammonium bromide (CTAB) as an outer-layer surfactant. Salt concentration of the aqueous solution affects the solubility of the organic solution droplets into the aqueous surfactant solution. At high NaCl concentration, over 60 % of extracted DNA was re-dispersed in the aqueous solution, while DNA was hardly dispersed in the solution without NaCl. Characterization of the resulting surfactant-DNA complexes was carried out by dynamic light scattering (DLS), and which showed that the average diameters were between 200-300 nm. This technique facilitates high encapsulation efficiency of DNA molecules into liposome, and the resultant lipid-DNA complex is nano-scale gene vector.

収録刊行物

• アジア・太平洋化学工学会議発表論文要旨集

  アジア・太平洋化学工学会議発表論文要旨集 2004 (0), 478-478, 2004

  公益社団法人 化学工学会

キーワード

• Vesicle
• Reverse micelle
• Gene vector

詳細情報

• CRID: 1390282680704465536
• NII論文ID: 130005052527
• DOI: 10.11491/apcche.2004.0.478.0
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可