Development of bFGF Sustained-Release Bioabsorbable Sponge and Enhancement of Angiogenesis in it

• Takei Takayuki

  Department of Chemical Engineering, Kyushu University, Japan

• Ijima Hiroyuki

  Department of Chemical Engineering, Kyushu University, Japan

• Sakai Shinji

  Department of Chemical Engineering, Kyushu University, Japan

• Ono Tsutomu

  Department of Chemical Engineering, Kyushu University, Japan

• Kawakami Koei

  Department of Chemical Engineering, Kyushu University, Japan

抄録

Hepatocyte transplantation using porous scaffolds is a potential therapy for both acute and chronic hepatic insufficiency and also for treatment of inborn errors of metabolism affecting liver. However, some authors reported that the hepatocytes located in the center of the scaffold died after transplantation due to insufficient oxygenation to the cells. No reports have been sufficiently addressed to resolve this problem. In this study, we fabricated a scaffold from poly (lacide acid) (PLA) coated with acidic gelatin. Basic fibroblast growth factor (bFGF) adsorbed onto the gelatin, would be released continuously and could induce angiogenesis in the scaffold in vivo. A combination of thickness and cell density in the scaffold was optimized so as to suppress the hepatocytes death in the center of the scaffold in the first stage of implantation. Further, a new centrifugal cell seeding method was developed to immobilize hepatocytes uniformly within the scaffold. The hepatocytes immobilized by the centrifugal cell seeding method in the bFGF-containing scaffold at the cell density optimized were implanted into mesentery of rat received a 70% hepatectomy. From observation of histological section of the scaffold after one week of implantation, it was confirmed that the living hepatocytes were uniformly distributed throughout the section, and exhibited PAS-positive indicating that they had a potential to store glycogen. This result indicates that the technique developed in this study was available for hepatocyte transplantation using a scaffold.

収録刊行物

• アジア・太平洋化学工学会議発表論文要旨集

  アジア・太平洋化学工学会議発表論文要旨集 2004 (0), 480-480, 2004

  公益社団法人 化学工学会

キーワード

• Hepatocyte transplantation
• Basic fibroblast growth factor
• Acidic gelatin
• Centrifugal cell seeding method

詳細情報

• CRID: 1390001205727758336
• NII論文ID: 130005052530
• DOI: 10.11491/apcche.2004.0.480.0
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可