Analysis of Cell Population Dynamics in Continuous Culture of <I>Escherichia coli</I>

• Yamada Tadashi

  Department of Bioinformatic Engineering, Osaka University, Japan

• Maeoka Kouhei

  Department of Biotechnology, Osaka University, Japan

• Kashiwagi Akiko

  Department of Biotechnology, Osaka University, Japan

• Nagahisa Keisuke

  Department of Bioinformatic Engineering, Osaka University, Japan

• Furusawa Chikara

  Department of Bioinformatic Engineering, Osaka University, Japan

• Shioya Suteaki

  Department of Biotechnology, Osaka University, Japan

• Yomo Tetsuya

  Department of Bioinformatic Engineering, Osaka University, Japan

• Shimizu Hiroshi

  Department of Bioinformatic Engineering, Osaka University, Japan

抄録

It has been generally believed that isogenic cells in the culture systems are homogeneous. However, recent studies pointed out that a heterogeneous population may emerge and exist in the system. In this study to investigate cell population dynamics in a continuous culture of Escherichia coli, a glutamine synthetase gene (glnA) deficient mutant of E. coli YMC21 was used as a host. The plasmid pKGN-EGFP, carrying the fusion gene of glnA and egfp (enhanced green fluorescent protein, clontech) was introduced into YMC21. A minimal medium, containing glucose and glutamate as sole carbon and nitrogen sources, respectively, was used for the continuous culture of the strain. The changes in GFP intensity and forward scattering on the log scale were measured by the flow cytometry, when glutamate concentration in the feeding medium was changed. In the steady state of the culture, the cell population seemed to consist of two subpopulations of GFP intensity. According to the decrease of glutamate concentration, cell density also decreased. During this phase, the subpopulation with a high GFP fluorescence intensity became the major group in the system. On the other hand, when the glutamate concentration increased, cell concentration increased too. During the phase, the subpopulation which had a low GFP fluorescence intensity became the major group in the system. Interestingly, the subpopulation with the high or low GFP fluorescence intensity was not maintained, and the original distribution of the cell population emerged after reaching the steady state. By clarifying the role of each cluster or population of cells, the cell-cell interaction and interconnection was discussed in the future experiments.

収録刊行物

• アジア・太平洋化学工学会議発表論文要旨集

  アジア・太平洋化学工学会議発表論文要旨集 2004 (0), 809-809, 2004

  公益社団法人 化学工学会

キーワード

• population dynamics
• continuous culture
• flow cytometory

詳細情報

• CRID: 1390001205732857984
• NII論文ID: 130005052895
• DOI: 10.11491/apcche.2004.0.809.0
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可