Quick Microfluidic Sequence-Selective DNA Detection using Non-Immobilized Probe

• Yamashita Kenichi

  Micro-space Chemistry Laboratory, National Institute of Advanced Science and Technology, Japan

• Yamaguchi Yoshiko

  Micro-space Chemistry Laboratory, National Institute of Advanced Science and Technology, Japan

• Miyazaki Masaya

  Micro-space Chemistry Laboratory, National Institute of Advanced Science and Technology, Japan

• Nakamura Hiroyuki

  Micro-space Chemistry Laboratory, National Institute of Advanced Science and Technology, Japan

• Shimizu Hazime

  Micro-space Chemistry Laboratory, National Institute of Advanced Science and Technology, Japan

• Maeda Hideaki

  Micro-space Chemistry Laboratory, National Institute of Advanced Science and Technology, Japan

抄録

Microfluidic system has superior controllability of fluidics. Herein we have developed a novel separation method for the complexes formed in microchannel, and application for DNA sensor. This method is based on the molecular sieving effect which is achieved by the superior controllability of fluidics. By the use of confocal fluorescence microscopy observation and computer simulation, we have confirmed that the laminar secondary flow at the curing part of microchannel enables this microfluidic separation. Also in this study, we have applied this molecular sieving effect for sequence-selective DNA sensor. As the result of this measurement, the response of complementary sequence target DNA was higher than non-complementary sequence. Moreover, in comparison with different length complementary sequence target, the response of longer target was higher. This method does not require immobilization of probe or target DNA, just simply inject solutions into microchannel and all reactions performed in the liquid phase. Such features might enable lowering the experimental error and difference in data by operators.

収録刊行物

• アジア・太平洋化学工学会議発表論文要旨集

  アジア・太平洋化学工学会議発表論文要旨集 2004 (0), 949-949, 2004

  公益社団法人 化学工学会

キーワード

• molecular sieving
• DNA sensor
• microreactor

詳細情報

• CRID: 1390282680709180416
• NII論文ID: 130005053050
• DOI: 10.11491/apcche.2004.0.949.0
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可