Defect of tropomyosin-related kinase B isotype expression in ovarian clear cell adenocarcinoma

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Author(s)

    • Goto Yumiko
    • Department of Obstetrics and Gynecology, Tokai University School of Medicine
    • Takeda Satoru
    • Department of Obstetrics and Gynecology, Juntendo University School of Medicine
    • Mikami Mikio
    • Department of Obstetrics and Gynecology, Tokai University School of Medicine
    • Kametani Yoshie
    • Department of Immunology, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine
    • Kikugawa Atsuko
    • Department of Obstetrics and Gynecology, Juntendo University School of Medicine
    • Tsuda Banri
    • Department of Breast and Endocrine Surgery, Tokai University School of Medicine
    • Miyazawa Masaki
    • Department of Obstetrics and Gynecology, Tokai University School of Medicine
    • Terao Yasuhisa
    • Department of Obstetrics and Gynecology, Juntendo University School of Medicine
    • Takekoshi Susumu
    • Department of Immunology, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine

Abstract

Tropomyosin-related kinase B (TrkB) is a functional signal molecule that correlates with cell survival and epithelial-mesenchymal transition (EMT), which is essential for the invasiveness of malignant cancer cells. While a truncated isoform of TrkB has a dominant negative effect, full-length TrkB with its tyrosine kinase domain is predicted to play a role in cancer progression. Because ovarian clear cell adenocarcinoma (CCA) shows worse prognosis compared to other cancer types, we investigated the correlation between TrkB isoforms and the progression of CCA. Ovarian adenocarcinoma and benign tumor samples were obtained from Tokai University Hospital and Juntendo University Hospital. These samples were examined for the TrkB expression of isotype-specific proteins and mRNAs by immunohistochemistry and domain-specific semi-quantitative reverse transcription polymerase chain reaction. While TrkB mRNA expression was detected in all of the ovarian tissues and TrkB protein expression was predominant in ovarian cancer tissues, the number of tissues expressing the tyrosine kinase-truncated isoforms (T-Shc or T1) decreased according to the clinical stage of CCA. Irregular isoforms were also observed in some CCA samples. The decrease in T-Shc and T1 were less obvious in mucinous adenocarcinoma and not observed in serous or endometrioid adenocarcinoma. Decreased expression of the truncated isoforms (T-Shc and T1) was associated with CCA progression. These results demonstrate that irregular expression of TrkB isoforms is a characteristic of CCA tissues. The unique TrkB expression profile may be useful for the diagnosis of CCA subtypes.

Journal

  • BioScience Trends

    BioScience Trends 8(2), 93-100, 2014

    International Research and Cooperation Association for Bio & Socio-Sciences Advancement

Codes

  • NII Article ID (NAID)
    130005054667
  • Text Lang
    ENG
  • ISSN
    1881-7815
  • Data Source
    J-STAGE 
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