Purification of innate immunostimulant from green tea using a silkworm muscle contraction assay

DOI 被引用文献9件 参考文献16件
  • Dhital Saphala
    Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
  • Hamamoto Hiroshi
    Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
  • Urai Makoto
    Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
  • Ishii Kenichi
    Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo
  • Sekimizu Kazuhisa
    Laboratory of Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo

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抄録

A polysaccharide was purified from a hot water extract of green tea leaves by measuring the immunostimulatory activity in silkworm larvae. Nuclear magnetic resonance and chemical analysis of acid hydrolysates revealed that the purified substance possessed a backbone containing polygalacturonic acids with methyl ester residues. Treatment with β-glucanase attenuated the muscle contraction activity of the purified sample, suggesting that the β-glucan structure, probably as a branched form, was required for its activity. The purified fraction stimulated the production of interleukin-6 by mouse peritoneal macrophages. These results suggest that measuring immunostimulation in silkworm larvae is useful for evaluating innate immunostimulants from various sources.

収録刊行物

  • Drug Discoveries & Therapeutics

    Drug Discoveries & Therapeutics 5 (1), 18-25, 2011

    特定非営利活動法人 バイオ＆ソーシャル・サイエンス推進国際研究交流会

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