Evaluation of innate immune stimulating activity of polysaccharides using a silkworm (<i>Bombyx mori</i>) muscle contraction assay

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著者

    • Fujiyuki Tomoko
    • Graduate School of Pharmaceutical Sciences, The University of Tokyo
    • Ishii Kenichi
    • Graduate School of Pharmaceutical Sciences, The University of Tokyo
    • Urai Makoto
    • Graduate School of Pharmaceutical Sciences, The University of Tokyo
    • Shibata Shoji
    • Graduate School of Pharmaceutical Sciences, The University of Tokyo
    • Sekimizu Kazuhisa
    • Graduate School of Pharmaceutical Sciences, The University of Tokyo|Genome Pharmaceuticals Institute Co., Ltd.

抄録

In silkworm larvae, the mature form of paralytic peptide (PP), an insect cytokine, is produced from pro-PP in association with activation of innate immune responses, resulting in slow muscle contraction. We utilized this reaction, muscle contraction in silkworms coupled with innate immunity stimulation, to quantitatively measure the innate immune stimulating activity of various natural polysaccharides. β-Glucan of <i>Gyrophora esculenta</i> (GE-3), fucoidan from sporophyll of <i>Undaria pinnatifida</i>, and curldan induced silkworm muscle contraction. We further demonstrated that GE-3 had therapeutic effects on silkworms infected by baculovirus. Based on these findings, we propose that the silkworm muscle contraction assay is useful for screening substances that stimulate innate immunity before evaluating therapeutic effectiveness in mammals.

収録刊行物

  • Drug Discoveries & Therapeutics

    Drug Discoveries & Therapeutics 6(2), 88-93, 2012

    特定非営利活動法人 バイオ&ソーシャル・サイエンス推進国際研究交流会

各種コード

  • NII論文ID(NAID)
    130005054745
  • 本文言語コード
    ENG
  • ISSN
    1881-7831
  • データ提供元
    J-STAGE 
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