Evaluation of innate immune stimulating activity of polysaccharides using a silkworm (Bombyx mori) muscle contraction assay
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- Fujiyuki Tomoko
- Graduate School of Pharmaceutical Sciences, The University of Tokyo
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- Hamamoto Hiroshi
- Graduate School of Pharmaceutical Sciences, The University of Tokyo
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- Ishii Kenichi
- Graduate School of Pharmaceutical Sciences, The University of Tokyo
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- Urai Makoto
- Graduate School of Pharmaceutical Sciences, The University of Tokyo
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- Kataoka Keiko
- Genome Pharmaceuticals Institute Co., Ltd.
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- Takeda Tadahiro
- Department of Sports Health, Nagoya Gakuin University
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- Shibata Shoji
- Graduate School of Pharmaceutical Sciences, The University of Tokyo
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- Sekimizu Kazuhisa
- Graduate School of Pharmaceutical Sciences, The University of Tokyo Genome Pharmaceuticals Institute Co., Ltd.
書誌事項
- タイトル別名
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- Evaluation of innate immune stimulating activity of polysaccharides using a silkworm (<i>Bombyx mori</i>) muscle contraction assay
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抄録
In silkworm larvae, the mature form of paralytic peptide (PP), an insect cytokine, is produced from pro-PP in association with activation of innate immune responses, resulting in slow muscle contraction. We utilized this reaction, muscle contraction in silkworms coupled with innate immunity stimulation, to quantitatively measure the innate immune stimulating activity of various natural polysaccharides. β-Glucan of Gyrophora esculenta (GE-3), fucoidan from sporophyll of Undaria pinnatifida, and curldan induced silkworm muscle contraction. We further demonstrated that GE-3 had therapeutic effects on silkworms infected by baculovirus. Based on these findings, we propose that the silkworm muscle contraction assay is useful for screening substances that stimulate innate immunity before evaluating therapeutic effectiveness in mammals.
収録刊行物
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- Drug Discoveries & Therapeutics
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Drug Discoveries & Therapeutics 6 (2), 88-93, 2012
特定非営利活動法人 バイオ＆ソーシャル・サイエンス推進国際研究交流会