多剤耐性緑膿菌の検出におけるクロモアガーMDRPスクリーン培地の基礎検討  [in Japanese] Fundamental assessment of the CHROMagar<sup>TM</sup> MDRP medium in the screening of multidrug-resistant <i>Pseudomonas aeruginosa</i>  [in Japanese]

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Author(s)

    • 馬場 尚志 BABA Hisashi
    • 金沢医科大学病院中央臨床検査部|金沢医科大学臨床感染症学 Center of Laboratory Medicine, Kanazawa Medical University Hospital|Department of Infectious Diseases, Kanazawa Medical University
    • 麻生 都 ASOU Miyako
    • 金沢医科大学病院中央臨床検査部 Center of Laboratory Medicine, Kanazawa Medical University Hospital
    • 森田 恵美 MORITA Emi
    • 金沢医科大学病院中央臨床検査部 Center of Laboratory Medicine, Kanazawa Medical University Hospital
    • 河村 佳江 KAWAMURA Kae
    • 金沢医科大学病院中央臨床検査部 Center of Laboratory Medicine, Kanazawa Medical University Hospital
    • 飯沼 由嗣 IINUMA Yoshitsugu
    • 金沢医科大学病院中央臨床検査部|金沢医科大学臨床感染症学 Center of Laboratory Medicine, Kanazawa Medical University Hospital|Department of Infectious Diseases, Kanazawa Medical University

Abstract

多剤耐性緑膿菌は,病院感染対策上最も重要な病原体の1つであり,確実な感染対策を行うためにも,迅速かつ効果的なスクリーニング法が求められている.今回我々は,臨床分離株のうちimipenem,ciprofloxacin,amikacinのいずれか1つ以上に耐性を示した緑膿菌31株と,同様の耐性を持つ他のグラム陰性桿菌30株を用い,クロモアガーMDRPスクリーン培地(MDRP培地)の有用性に関する基礎的検討を行った.結果は,緑膿菌では多剤耐性菌以外の株でも一部に発育が見られたものの,多剤耐性緑膿菌8株全てで青緑色のコロニー形成を認めた.一方,他の菌種は高度耐性株を含め多くの株で発育が抑制され,発育を認めた場合でもコロニーの色調から容易に緑膿菌との違いが判別できた.この結果から,最終的な判定には薬剤感受性検査の実施が不可欠であるものの,MDRP培地が多剤耐性緑膿菌のスクリーニング培地として高い有用性を持つ可能性が示唆された.

Multidrug-resistant <i>Pseudomonas aeruginosa</i> (MDRP) is one of the most important pathogens in hospital infection control. Rapid and efficient methods of detecting MDRP are needed because early recognition of MDRP colonization/infection is important in the prevention of MDRP nosocomial spread. We evaluated the fundamental performance of CHROMagar<sup>TM</sup> MDRP medium, a novel chromogenic agar medium, in the detection of MDRP. We cultured 31 <i>P. aeruginosa</i> and 30 other Gram-negative bacilli that were resistant to one or more of the following antibiotics on CHROMagar<sup>TM</sup> MDRP medium: imipenem, ciprofloxacin and amikacin. As a result, all 8 isolates of MDRP were observed as blue-green colonies on the medium, although some of the non-MDRP <i>P. aeruginosa</i> isolates also grew. Among the bacteria other than <i>P. aeruginosa</i>, 8 of 30 strains were recovered. The observed colonies were easily distinguished from that of <i>P. aeruginosa</i> by the difference of color. Thus, our data suggest that CHROMagar<sup>TM</sup> MDRP medium could be highly useful as a screening tool for detecting MDRP although antimicrobial susceptibility testing is necessary for confirmation.

Journal

  • Japanese Journal of Medical Technology

    Japanese Journal of Medical Technology 63(2), 226-231, 2014

    Japanese Association of Medical Technologists

Codes

  • NII Article ID (NAID)
    130005057055
  • Text Lang
    JPN
  • ISSN
    0915-8669
  • Data Source
    J-STAGE 
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