Fundamental assessment of the CHROMagar^TM MDRP medium in the screening of multidrug-resistant <i>Pseudomonas aeruginosa</i>

• MATSUURA Kaori

  Center of Laboratory Medicine, Kanazawa Medical University Hospital

• BABA Hisashi

  Center of Laboratory Medicine, Kanazawa Medical University Hospital Department of Infectious Diseases, Kanazawa Medical University

• ASOU Miyako

  Center of Laboratory Medicine, Kanazawa Medical University Hospital

• MORITA Emi

  Center of Laboratory Medicine, Kanazawa Medical University Hospital

• KANAYA Kazumi

  Center of Laboratory Medicine, Kanazawa Medical University Hospital

• KAWAMURA Kae

  Center of Laboratory Medicine, Kanazawa Medical University Hospital

• IINUMA Yoshitsugu

  Center of Laboratory Medicine, Kanazawa Medical University Hospital Department of Infectious Diseases, Kanazawa Medical University

Bibliographic Information

Other Title

• 多剤耐性緑膿菌の検出におけるクロモアガーMDRPスクリーン培地の基礎検討

Abstract

Multidrug-resistant Pseudomonas aeruginosa (MDRP) is one of the most important pathogens in hospital infection control. Rapid and efficient methods of detecting MDRP are needed because early recognition of MDRP colonization/infection is important in the prevention of MDRP nosocomial spread. We evaluated the fundamental performance of CHROMagar^TM MDRP medium, a novel chromogenic agar medium, in the detection of MDRP. We cultured 31 P. aeruginosa and 30 other Gram-negative bacilli that were resistant to one or more of the following antibiotics on CHROMagar^TM MDRP medium: imipenem, ciprofloxacin and amikacin. As a result, all 8 isolates of MDRP were observed as blue-green colonies on the medium, although some of the non-MDRP P. aeruginosa isolates also grew. Among the bacteria other than P. aeruginosa, 8 of 30 strains were recovered. The observed colonies were easily distinguished from that of P. aeruginosa by the difference of color. Thus, our data suggest that CHROMagar^TM MDRP medium could be highly useful as a screening tool for detecting MDRP although antimicrobial susceptibility testing is necessary for confirmation.

Journal

• Japanese Journal of Medical Technology

  Japanese Journal of Medical Technology 63 (2), 226-231, 2014

  Japanese Association of Medical Technologists

Keywords

• Multidrug-resistant <i>Pseudomonas aeruginosa</i>
• CHROMagar^TM
• MDRP medium

Details

• CRID: 1390001205741228800
• NII Article ID: 130005057055
• DOI: 10.14932/jamt.13-56
• ISSN: 21885346
• ISSN: 09158669
• Web Site: https://search.jamas.or.jp/link/ui/2014204762
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed