Multimodal Imaging of Living Cells with Multiplex Coherent Anti-stokes Raman Scattering (CARS), Third-order Sum Frequency Generation (TSFG) and Two-photon Excitation Fluorescence (TPEF) Using a Nanosecond White-light Laser Source

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  • SEGAWA Hiroki
    Department of Chemistry, School of Science, The University of Tokyo
  • OKUNO Masanari
    Department of Chemistry, Graduate School of Pure and Applied Sciences,University of Tsukuba
  • LEPROUX Philippe
    Xlim Research Institute, CNRS-University of Limoges
  • COUDERC Vincent
    Xlim Research Institute, CNRS-University of Limoges
  • OZAWA Takeaki
    Department of Chemistry, School of Science, The University of Tokyo
  • KANO Hideaki
    Institute of Applied Physics, University of Tsukuba Graduate School of Pure and Applied Sciences, University of Tsukuba

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The subnanosecond “white-light laser” source has been applied to multimodal, multiphoton, and multiplex spectroscopic imaging (M3 spectroscopic imaging) with coherent anti-Stokes Raman scattering (CARS), third-order sum frequency generation (TSFG), and two-photon excitation fluorescence (TPEF). As the proof-of-principle experiment, we performed simultaneous imaging of polystyrene beads with TSFG and TPEF. This technique is then applied to live cell imaging. Mouse L929 fibroblastic cells are clearly visualized by CARS, TSFG, and TPEF processes. M3 spectroscopic imaging provides various and unique cellular information with different image contrast based on each multiphoton process.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 31 (4), 299-305, 2015

    社団法人 日本分析化学会

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