Multi-point Scanning Two-photon Excitation Microscopy by Utilizing a High-peak-power 1042-nm Laser

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Author(s)

    • HIBI Terumasa HIBI Terumasa
    • Research Institute for Electronic Science, Hokkaido University|Graduate School of Information Science and Technology, Hokkaido University
    • MURATA Takashi [他] MURATA Takashi
    • Division of Evolutionary Biology, National Institute for Basic Biology|Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies)
    • WATANABE Hirotaka
    • Research Institute for Electronic Science, Hokkaido University|Graduate School of Information Science and Technology, Hokkaido University
    • KAWAKAMI Ryosuke
    • Research Institute for Electronic Science, Hokkaido University|Graduate School of Information Science and Technology, Hokkaido University
    • HASEBE Mitsuyasu
    • Division of Evolutionary Biology, National Institute for Basic Biology|Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies)
    • NEMOTO Tomomi
    • Research Institute for Electronic Science, Hokkaido University|Graduate School of Information Science and Technology, Hokkaido University

Abstract

The temporal resolution of a two-photon excitation laser scanning microscopy (TPLSM) system is limited by the excitation laser beam’s scanning speed. To improve the temporal resolution, the TPLSM system is equipped with a spinning-disk confocal scanning unit. However, the insufficient energy of a conventional Ti:sapphire laser source restricts the field of view (FOV) for TPLSM images to a narrow region. Therefore, we introduced a high-peak-power Yb-based laser in order to enlarge the FOV. This system provided three-dimensional imaging of a sufficiently deep and wide region of fixed mouse brain slices, clear four-dimensional imaging of actin dynamics in live mammalian cells and microtubule dynamics during mitosis and cytokinesis in live plant cells.

Journal

  • Analytical Sciences

    Analytical Sciences 31(4), 307-313, 2015

    Japan Society for Analytical Chemistry

Codes

  • NII Article ID (NAID)
    130005061532
  • NII NACSIS-CAT ID (NCID)
    AA10500785
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    0910-6340
  • NDL Article ID
    026322045
  • NDL Call No.
    Z54-F482
  • Data Source
    NDL  IR  J-STAGE 
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