An Enzyme-entrapped Agarose Gel for Visualization of Ischemia-induced L-Glutamate Fluxes in Hippocampal Slices in a Flow System

• TANAKA Kazuhisa

  Department of Chemistry, College of Humanities and Sciences, Nihon University

• SHOJI Atushi

  Tokyo University of Pharmacy and Life Sciences

• SUGAWARA Masao

  Department of Chemistry, College of Humanities and Sciences, Nihon University

抄録

An agarose gel slip containing L-glutamate oxidase (GluOx), horseradish peroxidase (HRP) and a dye DA-64 is proposed as a tool for visualizing ischemia-induced L-glutamate release in hippocampal slices in a flow system. The agarose slip with a detection limit of 6.0 ± 0.8 μmol L⁻¹ for L-glutamate enabled us to visualize L-glutamate fluxes in a flow system. The leak of a dye from the agarose gel was negligible and a diffusion blur due to spreading of Bindshedler’s Green (BG) within the gel was suppressed. Monitoring the time-dependent change of ischemia-induced L-glutamate fluxes at neuronal regions CA1, DG and CA3 of hippocampal slices is demonstrated.

収録刊行物

• Analytical Sciences

  Analytical Sciences 31 (4), 321-325, 2015

  社団法人 日本分析化学会

キーワード

• Enzyme-entrapped agarose gel
• imaging of L-glutamate
• hippocampal slice
• ischemia

詳細情報

• CRID: 1390282679236782336
• NII論文ID: 130005061534
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.31.321
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 026322064
• PubMed: 25864676
• Web Site: http://id.ndl.go.jp/bib/026322064
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026322064
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.31.321.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.31.321/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/31/4/31_321/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2015386058
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
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