Molecular Characterization of Viable Legionella spp. in Cooling Tower Water Samples by Combined Use of Ethidium Monoazide and PCR

• Inoue Hiroaki

  Tsukuba Research Laboratories, Aquas Corporation Ibaraki University College of Agriculture

• Fujimura Reiko

  Ibaraki University College of Agriculture

• Agata Kunio

  Tsukuba Research Laboratories, Aquas Corporation

• Ohta Hiroyuki

  Ibaraki University College of Agriculture

書誌事項

タイトル別名

• Molecular Characterization of Viable <i>Legionella</i> spp. in Cooling Tower Water Samples by Combined Use of Ethidium Monoazide and PCR

抄録

Viable Legionella spp. in environmental water samples were characterized phylogenetically by a clone library analysis combining the use of ethidium monoazide and quantitative PCR. To examine the diversity of Legionella spp., six cooling tower water samples and three bath water samples were collected and analyzed. A total of 617 clones were analyzed for their 16S rRNA gene sequences and classified into 99 operational taxonomic units (OTUs). The majority of OTUs were not clustered with currently described Legionella spp., suggesting the wide diversity of not-yet-cultured Legionella groups harbored in cooling tower water environments.

収録刊行物

• Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ

  Ｍｉｃｒｏｂｅｓ　ａｎｄ　ｅｎｖｉｒｏｎｍｅｎｔｓ 30 (1), 108-112, 2015

  日本微生物生態学会 / 日本土壌微生物学会 / Taiwan Society of Microbial Ecology / 植物微生物研究会 / 極限環境微生物学会

キーワード

• clone library
• Ethidium monoazide (EMA)
• <i>Legionella</i>
• quantitative polymerase chain reaction (qPCR)
• Viable but nonculturable (VBNC)

詳細情報

• CRID: 1390282679322906496
• NII論文ID: 130005061717
• NII書誌ID: AA11551577
• DOI: 10.1264/jsme2.me14115
• ISSN: 13474405
• ISSN: 13426311
• NDL書誌ID: 026292589
• PubMed: 25736979
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I026292589
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可